Full T D, Jung D O, Madigan M T
Department of Microbiology, Southern Illinois University, Carbondale, IL 62901, USA.
Lett Appl Microbiol. 2006 Oct;43(4):377-84. doi: 10.1111/j.1472-765X.2006.01981.x.
To isolate and characterize bacteria from nature capable of producing poly-beta-hydroxyalkanoates in high yields from soy molasses oligosaccharides.
Several strains of bacteria were obtained from enrichment cultures employing raffinose as major carbon source and inoculated with soybean field soil, lake sediment, or lake water. Many of the isolates were Bacillus species and produced polyhydroxyalkanoates (PHAs) to high yield. The raffinose-degrading isolates produced endospores, were highly saccharolytic, and both respired and fermented a variety of mono-, di-, tri- and tetrasaccharides. Strain CL1 produced 90% of cell dry mass as PHA from various sugars, including raffinose, and did so without requiring a nutrient limitation.
Strain CL1 could be the catalyst for an industrial fermentation converting soy molasses and other waste carbohydrates to PHAs. The properties of this organism that make it ideally suited for such a fermentation include (i) its ability to use a wide variety of plant-associated carbohydrates as PHA feedstocks; (ii) its rapid growth; (iii) its ability to grow under anoxic conditions; and (iv) its ability to produce spores.
This is the first report of bacteria capable of making biodegradable plastics to high yield from soy molasses oligosaccharides.
从自然界中分离并鉴定能够利用大豆糖蜜低聚糖高产聚-β-羟基链烷酸酯的细菌。
以棉子糖作为主要碳源，用大豆田土壤、湖泊沉积物或湖水接种，从富集培养物中获得了几株细菌。许多分离菌株为芽孢杆菌属，能高产聚羟基链烷酸酯（PHA）。降解棉子糖的分离菌株能产生内生孢子，具有高度的糖分解能力，能呼吸并发酵多种单糖、二糖、三糖和四糖。CL1菌株能利用包括棉子糖在内的多种糖类，将90%的细胞干重转化为PHA，且无需营养限制。
CL1菌株可能是将大豆糖蜜和其他废弃碳水化合物转化为PHA的工业发酵的催化剂。该菌株适合这种发酵的特性包括：（i）能够利用多种与植物相关的碳水化合物作为PHA原料；（ii）生长迅速；（iii）能在缺氧条件下生长；（iv）能产生孢子。
这是关于能够利用大豆糖蜜低聚糖高产可生物降解塑料的细菌的首次报道。
