Li Z-Y, Liu Y
Marine Biotechnology Laboratory, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, PR China.
Lett Appl Microbiol. 2006 Oct;43(4):410-6. doi: 10.1111/j.1472-765X.2006.01976.x.
The aim of this study was to investigate the bacterial diversity associated with the sponge Craniella australiensis using a molecular strategy and isolating Actinomycetes with antimicrobial potentials.
The bacterial diversity associated with South China Sea sponge C. austrialiensis was assessed using a 16S rDNA clone library alongside restriction fragment length polymorphism and phylogenetic analysis. It was found that the C. austrialiensis-associated bacterial community consisted of alpha, beta and gamma-Proteobacteria, Firmicutes, Bacteroidetes as well as Actinobacterium. Actinomycetes were isolated successfully using seawater medium with sponge extracts. According to the BLAST and phylogenetic analysis based on about 600-bp 16S rDNA sequences, 11 of the representative 23 isolates closely matched the Streptomyces sp. while the remaining 12 matched the Actinomycetales. Twenty Actinomycetes have antimicrobial potentials, of which 15 are found to possess broad-spectrum antimicrobial potentials.
The sponge C. austrialiensis-associated bacterial community is very abundant including Proteobacteria, Firmicutes, Bacteroidetes and Actinobacterium while Actinomycetes is not predominant. Artificial seawater medium with sponge extracts is suitable for Actinomycetes isolation. Most of the isolated C. austrialiensis-associated Actinomycetes have a broad spectrum of antimicrobial activity.
This study revealed the diversity of the bacterial community and the isolated Actinomycetes with antimicrobial potentials associated with sponge C. australiensis.
本研究旨在采用分子策略调查与海绵澳大利亚颅海绵相关的细菌多样性，并分离具有抗菌潜力的放线菌。
利用16S rDNA克隆文库以及限制性片段长度多态性和系统发育分析，评估了与中国南海海绵澳大利亚颅海绵相关的细菌多样性。结果发现，与澳大利亚颅海绵相关的细菌群落由α、β和γ-变形菌门、厚壁菌门、拟杆菌门以及放线菌纲组成。使用含有海绵提取物的海水培养基成功分离出放线菌。根据基于约600 bp 16S rDNA序列的BLAST和系统发育分析，23株代表性菌株中有11株与链霉菌属密切匹配，其余12株与放线菌目匹配。20株放线菌具有抗菌潜力，其中15株具有广谱抗菌潜力。
与海绵澳大利亚颅海绵相关的细菌群落非常丰富，包括变形菌门、厚壁菌门、拟杆菌门和放线菌纲，而放线菌并不占主导地位。含有海绵提取物的人工海水培养基适合放线菌的分离。大多数分离出的与澳大利亚颅海绵相关的放线菌具有广谱抗菌活性。
本研究揭示了与海绵澳大利亚颅海绵相关的细菌群落的多样性以及分离出的具有抗菌潜力的放线菌。
