O'Byrne E M, Blancuzzi V J, Wilson D E, Wong M, Peppard J, Simke J P, Jeng A Y
Research Department, CIBA-GEIGY Corporation, Summit NJ 07901.
Int J Tissue React. 1990;12(1):11-4.
Inflammation was induced by intra-articular injection of 100 ng recombinant human interleukin-1 alpha (rhIL-1) into rabbit knees. Substance P (SP) and prostaglandin E2 (PGE2) were measured by radioimmunoassay (RIA) in the joint fluid at 4, 24 and 48 h after rhIL-1 injection. SP was increased by 4 h, further increased at 24 h and remained elevated at 48 h. PGE2 concentration was highest at 4 h and remained elevated at 48 h after rhIL-1 injection. Because of the proinflammatory activities of SP and PGE2, our studies suggest that the elevated SP and PGE2, in the joint may amplify or sustain an initial receptor-mediated inflammatory response to IL-1.
通过向兔膝关节腔内注射100纳克重组人白细胞介素-1α（rhIL-1）诱导炎症。在注射rhIL-1后4小时、24小时和48小时，通过放射免疫分析（RIA）测定关节液中的P物质（SP）和前列腺素E2（PGE2）。SP在4小时时升高，在24小时时进一步升高，并在48小时时保持升高。rhIL-1注射后4小时PGE2浓度最高，并在48小时时保持升高。由于SP和PGE2的促炎活性，我们的研究表明，关节中升高的SP和PGE2可能会放大或维持对IL-1的初始受体介导的炎症反应。
