Gaymann W, Falke N
Sektion Elektronenmikroskopie, Universität Ulm, F.R.G.
Neurosci Lett. 1990 Apr 20;112(1):114-9. doi: 10.1016/0304-3940(90)90332-4.
High concentrations of immunoreactive galanin-like material in rat hypothalamus, median eminence and neurohypophysis have been reported in the literature suggesting a regulatory role of galanin on hormone release from the anterior and posterior lobe of the pituitary. We studied binding of iodinated galanin to crude membrane preparations from porcine anterior hypothalamus, anterior and neurointermediate lobe of the hypophysis. In contrast to the hypothalamus where specific binding of 125I-galanin was found, there was no displaceable galanin binding in membranes of the anterior or neurointermediate lobe of porcine pituitaries. Effects of galanin on oxytocin and vasopressin release were investigated using isolated neurosecretory endings from rat neurohypophyses. Galanin had no detectable effect on the release of oxytocin or vasopressin.
文献报道大鼠下丘脑、正中隆起和神经垂体中存在高浓度的免疫反应性甘丙肽样物质，提示甘丙肽对垂体前叶和后叶激素释放具有调节作用。我们研究了碘化甘丙肽与猪下丘脑前部、垂体前叶和神经中间叶粗制膜制剂的结合情况。与发现125I-甘丙肽特异性结合的下丘脑不同，猪垂体前叶或神经中间叶的膜中没有可置换的甘丙肽结合。使用大鼠神经垂体分离的神经分泌末梢研究了甘丙肽对催产素和加压素释放的影响。甘丙肽对催产素或加压素的释放没有可检测到的影响。
