Li Jianjun, Richards James C
Institute for Biological Sciences, National Research Council, Ottawa, ON, Canada K1A 0R6.
Mass Spectrom Rev. 2007 Jan-Feb;26(1):35-50. doi: 10.1002/mas.20105.
Capillary electrophoresis (CE) is a high-resolution technique for the separation of complex biological mixtures and has been widely applied to biological analyses. The coupling of capillary electrophoresis with mass spectrometry (MS) provides a powerful approach for rapid identification of target analytes present at trace levels in biological matrices, and for structural characterization of complex biomolecules. Here we review the analytical potential of combined capillary electrophoresis electrospray mass spectrometry (CE-MS) for the analysis of bacterial lipopolysaccharides (LPS). This hyphened methodology facilitates the determination of closely related LPS glycoform and isoform families by exploiting differences in their unique molecular conformations and ionic charge distributions by electrophoretic separation. On-line CE-MS also provides an additional avenue to improve detection limits, which has been successfully applied to directly probe oligosaccharide LPS glycoform populations of bacteria isolated from infected animal models without the need for further passage.
毛细管电泳（CE）是一种用于分离复杂生物混合物的高分辨率技术，已广泛应用于生物分析。毛细管电泳与质谱（MS）联用为快速鉴定生物基质中痕量水平的目标分析物以及复杂生物分子的结构表征提供了一种强大的方法。在此，我们综述了毛细管电泳-电喷雾质谱联用（CE-MS）在分析细菌脂多糖（LPS）方面的分析潜力。这种联用方法通过利用电泳分离中密切相关的LPS糖型和亚型家族独特的分子构象和离子电荷分布差异，有助于对其进行测定。在线CE-MS还提供了另一种提高检测限的途径，已成功应用于直接探测从感染动物模型中分离出的细菌的寡糖LPS糖型群体，而无需进一步传代。
