Morales-Cid Gabriel, Simonet Bartolomé M, Cárdenas Soledad, Valcárcel Miguel
Department of Analytical Chemistry, University of Córdoba, Córdoba, Spain.
Electrophoresis. 2008 May;29(10):2033-40. doi: 10.1002/elps.200700565.
A substantial demand currently exists for analytical methods affording the determination of very low concentrations of analytes in complex matrices, such as those of environmental and biological samples, as simply as possible. However, the pretreatment of complex samples, which is unavoidable prior to CE-MS analysis, is usually complicated and time-consuming. In this work, we used voltage-assisted SPE for the first time as an alternative to conventional treatments for preconcentrating and purifying analytes. To this end, we used a simple flow system coupled on-line to CE-MS equipment. The system is quite robust and provides reproducible peak areas (the precision ranges from 2.5 to 3.8%). Also, it provides increased sensitivity affording the determination of trace amounts (nanogram per liter levels) of analytes in only a few milliliters of sample. The proposed system was applied to the determination of members of two compound families (viz. tetracyclines and amines).
目前，对于能够尽可能简单地测定复杂基质（如环境和生物样品）中极低浓度分析物的分析方法存在大量需求。然而，在CE-MS分析之前不可避免的复杂样品预处理通常既复杂又耗时。在这项工作中，我们首次使用电压辅助固相萃取作为传统处理方法的替代方法，用于预富集和纯化分析物。为此，我们使用了一个简单的流动系统与CE-MS设备在线联用。该系统相当稳健，提供可重现的峰面积（精密度范围为2.5%至3.8%）。此外，它还提高了灵敏度，仅需几毫升样品就能测定痕量（纳克每升水平）的分析物。所提出的系统应用于两个化合物家族（即四环素和胺类）成员的测定。
