Dong Jian, Solntsev Kyril M, Tolbert Laren M
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA.
J Am Chem Soc. 2006 Sep 20;128(37):12038-9. doi: 10.1021/ja063128a.
The solvatochromic behavior of the green fluorescence protein (GFP) chromophore (p-hydroxybenzylideneimidazolone, p-HBDI) and its derivatives (p-methoxybenzylideneimidazolone, p-MeOBDI, and N-methyl-p-hydroxybenzylideneimidazolonium iodide, p-HBDIMe+) was studied using UV-vis-absorption spectroscopy in a wide array of solvents. The relative contribution of specific (polarity) vs nonspecific (hydrogen-bonding) solvation to the absorbance spectra was studied. On the basis of these data, we discuss the nature of the absorption peak of the protonated and deprotonated forms of the wild-type GFP.
利用紫外可见吸收光谱法，在多种溶剂中研究了绿色荧光蛋白（GFP）发色团（对羟基苄叉咪唑啉酮，p-HBDI）及其衍生物（对甲氧基苄叉咪唑啉酮，p-MeOBDI，以及N-甲基-对羟基苄叉咪唑鎓碘化物，p-HBDIMe+）的溶剂化显色行为。研究了特异性（极性）与非特异性（氢键）溶剂化对吸收光谱的相对贡献。基于这些数据，我们讨论了野生型GFP质子化和去质子化形式吸收峰的性质。
