Ariel N, Lehrer S, Elhanaty E, Sabo T, Brodt P, Lachmi B, Katz D, Levin R, Grosfeld H, Velan B
Department of Biochemistry, Israel Institute for Biological Research, Ness Ziona.
Arch Virol. 1990;113(1-2):99-106. doi: 10.1007/BF01318358.
A set of 41 overlapping peptides, representing the complete sequence of SFV-E2 protein were synthesized and analyzed in the ELISA test against murine anti-SFV sera. No single peptide was recognized by all antisera. Eight peptides were found to be highly reactive with hyperimmune anti-SFV sera. Six out of the eight peptide sequences coincide with the most hydrophilic regions of SFV-E2. Out of these, four peptides (amino acid positions 16-35, 61-80, 166-185, 286-305) that contain the least number of alphavirus conserved residues were selected. This panel constitutes the minimal number of peptides necessary and sufficient for specific recognition of hyperimmune mouse anti-SFV sera.
合成了一组代表SFV-E2蛋白完整序列的41个重叠肽，并在针对鼠抗SFV血清的ELISA试验中进行分析。没有一个肽能被所有抗血清识别。发现8个肽与超免疫抗SFV血清具有高反应性。这8个肽序列中有6个与SFV-E2的最亲水区域一致。其中，选择了4个包含最少甲病毒保守残基的肽（氨基酸位置16-35、61-80、166-185、286-305）。该肽组构成了特异性识别超免疫小鼠抗SFV血清所需的最少且足够数量的肽。
