使用代表呼吸道合胞病毒大糖蛋白G的合成肽进行定点血清学检测。
Site-directed serology with synthetic peptides representing the large glycoprotein G of respiratory syncytial virus.
作者信息
Norrby E, Mufson M A, Alexander H, Houghten R A, Lerner R A
出版信息
Proc Natl Acad Sci U S A. 1987 Sep;84(18):6572-6. doi: 10.1073/pnas.84.18.6572.
Abstract
A set of 23 nested 15-amino-acid-long peptides with overlaps of 5 amino acids, representing the complete extramembranous part of the large glycoprotein G of respiratory syncytial (RS) virus, was analyzed in ELISA against different sera containing virus-specific antibodies. Seven of the peptides reacted with rabbit hyperimmune sera against purified virions. In contrast, only one of these seven peptides reacted with murine monoclonal antibodies specific for G. In connection with RS virus infections in humans, increase of antibody titers against three peptides was found in about one-third of the cases. These three peptides were included among those identified by both murine and rabbit antibodies. The present findings may open possibilities for site-directed clinical serology in the case of RS virus infections.
摘要
一组23个嵌套的、长度为15个氨基酸且有5个氨基酸重叠的肽段，代表呼吸道合胞（RS）病毒大糖蛋白G的完整膜外部分，针对含有病毒特异性抗体的不同血清进行了ELISA分析。其中七个肽段与抗纯化病毒粒子的兔超免疫血清发生反应。相比之下，这七个肽段中只有一个与针对G的鼠单克隆抗体发生反应。关于人类RS病毒感染，约三分之一的病例中发现针对三种肽段的抗体滴度升高。这三种肽段包含在鼠抗体和兔抗体都识别的肽段之中。目前的研究结果可能为RS病毒感染情况下的定点临床血清学研究开辟可能性。
相似文献
1
Site-directed serology with synthetic peptides representing the large glycoprotein G of respiratory syncytial virus.使用代表呼吸道合胞病毒大糖蛋白G的合成肽进行定点血清学检测。
Proc Natl Acad Sci U S A. 1987 Sep;84(18):6572-6. doi: 10.1073/pnas.84.18.6572.
2
Antibodies to respiratory syncytial virus polypeptides and their significance in human infection.呼吸道合胞病毒多肽抗体及其在人类感染中的意义。
J Gen Virol. 1983 Sep;64 (Pt 9):1867-76. doi: 10.1099/0022-1317-64-9-1867.
3
Strain-specific serum antibody responses in infants undergoing primary infection with respiratory syncytial virus.初次感染呼吸道合胞病毒的婴儿的毒株特异性血清抗体反应。
J Infect Dis. 1988 Apr;157(4):640-7. doi: 10.1093/infdis/157.4.640.
4
A subgroup-specific antigenic site in the G protein of respiratory syncytial virus forms a disulfide-bonded loop.呼吸道合胞病毒G蛋白中的一个亚组特异性抗原位点形成一个二硫键连接的环。
J Virol. 1990 Oct;64(10):5143-8. doi: 10.1128/JVI.64.10.5143-5148.1990.
5
Preparation and characterization of monoclonal antibodies directed against five structural components of human respiratory syncytial virus subgroup B.针对人呼吸道合胞病毒B亚组五种结构成分的单克隆抗体的制备与鉴定
J Gen Virol. 1987 Dec;68 ( Pt 12):3125-35. doi: 10.1099/0022-1317-68-12-3125.
6
Characterization of two antigenic sites recognized by neutralizing monoclonal antibodies directed against the fusion glycoprotein of human respiratory syncytial virus.针对人呼吸道合胞病毒融合糖蛋白的中和性单克隆抗体所识别的两个抗原位点的特性分析
J Gen Virol. 1992 Sep;73 ( Pt 9):2225-34. doi: 10.1099/0022-1317-73-9-2225.
7
Conformational constraints of conserved neutralizing epitopes from a major antigenic area of human respiratory syncytial virus fusion glycoprotein.人呼吸道合胞病毒融合糖蛋白主要抗原区域保守中和表位的构象限制
J Gen Virol. 1993 Dec;74 ( Pt 12):2567-77. doi: 10.1099/0022-1317-74-12-2567.
8
Immunoblot analysis of the human antibody response to respiratory syncytial virus infection.人对呼吸道合胞病毒感染的抗体反应的免疫印迹分析。
J Gen Virol. 1987 May;68 ( Pt 5):1267-75. doi: 10.1099/0022-1317-68-5-1267.
9
The response of infants with bronchiolitis to the proteins of respiratory syncytial virus.毛细支气管炎婴儿对呼吸道合胞病毒蛋白质的反应。
J Gen Virol. 1988 Jun;69 ( Pt 6):1229-39. doi: 10.1099/0022-1317-69-6-1229.
10
Antibody response to respiratory syncytial virus structural proteins in children with acute respiratory syncytial virus infection.急性呼吸道合胞病毒感染儿童对呼吸道合胞病毒结构蛋白的抗体反应。
J Virol. 1985 Mar;53(3):976-9. doi: 10.1128/JVI.53.3.976-979.1985.
引用本文的文献
1
Genetic Diversity of Human Respiratory Syncytial Virus during COVID-19 Pandemic in Yaoundé, Cameroon, 2020-2021.2020 - 2021年喀麦隆雅温得新冠疫情期间人类呼吸道合胞病毒的遗传多样性
Microorganisms. 2024 May 8;12(5):952. doi: 10.3390/microorganisms12050952.
2
Disordered epitopes as peptide vaccines.作为肽疫苗的无序表位
Pept Sci (Hoboken). 2018 May;110(3):e24067. doi: 10.1002/pep2.24067. Epub 2018 Apr 14.
3
Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011-2013.2011 - 2013年连续三个流行季节在喀麦隆南部急性呼吸道感染儿童中分离出的人类呼吸道合胞病毒的遗传多样性。
Trop Med Health. 2018 Apr 3;46:7. doi: 10.1186/s41182-018-0088-7. eCollection 2018.
4
Antigenic Fingerprinting following Primary RSV Infection in Young Children Identifies Novel Antigenic Sites and Reveals Unlinked Evolution of Human Antibody Repertoires to Fusion and Attachment Glycoproteins.幼儿初次感染呼吸道合胞病毒后的抗原指纹分析确定了新的抗原位点，并揭示了人类抗体库对融合糖蛋白和附着糖蛋白的非关联进化。
PLoS Pathog. 2016 Apr 21;12(4):e1005554. doi: 10.1371/journal.ppat.1005554. eCollection 2016 Apr.
5
Sequencing and analysis of globally obtained human respiratory syncytial virus A and B genomes.全球范围内获取的人呼吸道合胞病毒A和B基因组的测序与分析。
PLoS One. 2015 Mar 20;10(3):e0120098. doi: 10.1371/journal.pone.0120098. eCollection 2015.
6
A novel rabbit immunospot array assay on a chip allows for the rapid generation of rabbit monoclonal antibodies with high affinity.一种新型的芯片上兔免疫斑点分析检测方法可以快速产生高亲和力的兔单克隆抗体。
PLoS One. 2012;7(12):e52383. doi: 10.1371/journal.pone.0052383. Epub 2012 Dec 26.
7
Humoral response to the central unglycosylated region of the respiratory syncytial virus attachment protein.呼吸道合胞病毒附着蛋白中心无糖基化区域的体液反应。
Vaccine. 2010 Aug 31;28(38):6242-6. doi: 10.1016/j.vaccine.2010.07.011. Epub 2010 Jul 23.
8
Identification of and human serum reactogenicity to neutralizing epitopes within the central unglycosylated region of the respiratory syncytial virus attachment protein.呼吸道合胞病毒附着蛋白中央无糖基化区域内中和表位的鉴定及其与人血清的反应原性
Clin Vaccine Immunol. 2010 Apr;17(4):695-7. doi: 10.1128/CVI.00432-09. Epub 2010 Feb 17.
9
Subgroup prevalence and genotype circulation patterns of human respiratory syncytial virus in Belgium during ten successive epidemic seasons.连续十个流行季节期间比利时人呼吸道合胞病毒的亚组流行率和基因型传播模式
J Clin Microbiol. 2007 Sep;45(9):3022-30. doi: 10.1128/JCM.00339-07. Epub 2007 Jul 3.
10
Genetic variability of the G glycoprotein gene of human metapneumovirus.人偏肺病毒G糖蛋白基因的遗传变异性
J Clin Microbiol. 2004 Aug;42(8):3532-7. doi: 10.1128/JCM.42.8.3532-3537.2004.
本文引用的文献
1
Risk of respiratory syncytial virus infection for infants from low-income families in relationship to age, sex, ethnic group, and maternal antibody level.低收入家庭婴儿感染呼吸道合胞病毒的风险与年龄、性别、种族及母体抗体水平的关系。
J Pediatr. 1981 May;98(5):708-15. doi: 10.1016/s0022-3476(81)80829-3.
2
Immunochemical identification of viral and nonviral proteins of the respiratory syncytial virus virion.呼吸道合胞病毒病毒粒子的病毒蛋白和非病毒蛋白的免疫化学鉴定。
Infect Immun. 1982 Jul;37(1):243-9. doi: 10.1128/iai.37.1.243-249.1982.
3
4
Classification of hybridomas to respiratory syncytial virus glycoproteins.针对呼吸道合胞病毒糖蛋白的杂交瘤分类
Proc Soc Exp Biol Med. 1982 Dec;171(3):266-71. doi: 10.3181/00379727-171-41509.
5
Purification and characterization of GP90, one of the envelope glycoproteins of respiratory syncytial virus.呼吸道合胞病毒包膜糖蛋白之一GP90的纯化与特性分析
J Gen Virol. 1984 Apr;65 ( Pt 4):761-7. doi: 10.1099/0022-1317-65-4-761.
6
Protection from respiratory syncytial virus infection in cotton rats by passive transfer of monoclonal antibodies.通过单克隆抗体的被动转移保护棉鼠免受呼吸道合胞病毒感染。
Infect Immun. 1984 Feb;43(2):756-8. doi: 10.1128/iai.43.2.756-758.1984.
7
Antibodies of predetermined specificity in biology and medicine.生物学和医学中具有预定特异性的抗体。
Adv Immunol. 1984;36:1-44. doi: 10.1016/s0065-2776(08)60898-6.
8
Antibodies against a synthetic peptide identify the Epstein-Barr virus-determined nuclear antigen.针对合成肽的抗体可识别爱泼斯坦-巴尔病毒确定的核抗原。
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4652-6. doi: 10.1073/pnas.81.15.4652.
9
Monoclonal antibodies protect against respiratory syncytial virus infection in mice.单克隆抗体可保护小鼠免受呼吸道合胞病毒感染。
Immunology. 1984 May;52(1):137-42.
10
Location and chemical synthesis of a pre-S gene coded immunodominant epitope of hepatitis B virus.乙型肝炎病毒前S基因编码的免疫显性表位的定位与化学合成
Science. 1984 Apr 27;224(4647):392-5. doi: 10.1126/science.6200931.
Zotero 插件