Deguchi Y, Shibata N, Kishimoto S
Third Department of Internal Medicine, Osaka University School of Medicine, Japan.
Clin Exp Immunol. 1990 Aug;81(2):311-4. doi: 10.1111/j.1365-2249.1990.tb03336.x.
The expression of tumour necrosis factor-alpha (TNF-alpha) gene in peripheral blood mononuclear cells from patients with systemic vasculitis, periarteritis nodosa and Wegener's granulomatosis, has been studied by RNA dot blot and Northern blot assays. We further examined the transcriptional level of TNF-alpha gene in peripheral blood mononuclear cells from these patients by performing nuclear run on transcription assay. We demonstrate enhanced TNF-alpha gene expression in mononuclear cells from these patients compared with healthy subjects and patients with bronchial asthma. These findings suggest that the enhanced transcription of TNF-alpha gene in peripheral blood mononuclear cells from patients with systemic vasculitis may be involved in the pathophysiology/pathogenesis of these diseases by cytokine dysregulation.
通过RNA斑点印迹法和Northern印迹法，研究了系统性血管炎、结节性多动脉炎和韦格纳肉芽肿患者外周血单个核细胞中肿瘤坏死因子-α（TNF-α）基因的表达。我们通过进行核转录分析，进一步检测了这些患者外周血单个核细胞中TNF-α基因的转录水平。我们证明，与健康受试者和支气管哮喘患者相比，这些患者单核细胞中TNF-α基因表达增强。这些发现表明，系统性血管炎患者外周血单个核细胞中TNF-α基因转录增强，可能通过细胞因子失调参与这些疾病的病理生理学/发病机制。
