Erickson P A, Fisher S K
Neuroscience Research Institute, University of California, Santa Barbara 93106.
Exp Eye Res. 1990 Aug;51(2):145-52. doi: 10.1016/0014-4835(90)90066-4.
We have determined the pattern of RNA labeling (uridine incorporation) in the normal retina of the domestic cat. One eye in each of eight cats was labeled by injecting [3H]uridine into the vitreous cavity. Two of the labeled eyes had the lens and vitreous removed 10 days before labeling. Three additional animals received intravenous (i.v.) injections of [3H]uridine. All animals were injected 4 hr into the light period and fixed 24 hr later; then the retinas were divided into quadrants (ST = superior temporal, SN superior nasal, IT = inferior temporal, and IN = inferior nasal). The ST quadrant contains the area centralis and the SN quadrant the optic nerve head. Autoradiograms were prepared from plastic sections 1 micron thick taken near the centre of each quadrant. In animals receiving intravitreal [3H]uridine, the ganglion cells and the inner and outer nuclear layers (INL; ONL) were heavily labeled; the synaptic layers and the retinal pigment epithelium (RPE) were very lightly labeled. Amacrines were the heaviest labeled cells in the INL; cones were more heavily labeled than rods in the ONL. This finding indicates that amacrines and cone photoreceptors may be synthesizing RNA more actively than other retinal neurons. In animals receiving intravenous [3H]uridine the pattern of labeling was the same as above except that the RPE was heavily labeled. Because cells in the ST quadrant appeared to be more heavily labeled than the same cell types in the other retinal quadrants, silver grains over the ONL in each quadrant were counted as grains micron -2 or grains per rod nucleus.(ABSTRACT TRUNCATED AT 250 WORDS)
我们已经确定了家猫正常视网膜中RNA标记（尿苷掺入）的模式。给八只猫中的每只猫的一只眼睛通过向玻璃体腔注射[3H]尿苷进行标记。其中两只标记的眼睛在标记前10天摘除了晶状体和玻璃体。另外三只动物接受了静脉注射[3H]尿苷。所有动物均在光照期4小时时注射，并在24小时后固定；然后将视网膜分成象限（ST = 颞上，SN = 鼻上，IT = 颞下，IN = 鼻下）。ST象限包含中央凹区域，SN象限包含视神经乳头。从每个象限中心附近切取的1微米厚的塑料切片制备放射自显影片。在接受玻璃体内[3H]尿苷注射的动物中，神经节细胞以及内核层和外核层（INL；ONL）被大量标记；突触层和视网膜色素上皮（RPE）标记非常浅。无长突细胞是INL中标记最重的细胞；在ONL中，视锥细胞比视杆细胞标记更重。这一发现表明，无长突细胞和视锥光感受器可能比其他视网膜神经元更活跃地合成RNA。在接受静脉注射[3H]尿苷的动物中，标记模式与上述相同，只是RPE被大量标记。由于ST象限中的细胞似乎比其他视网膜象限中相同类型的细胞标记更重，因此对每个象限中ONL上的银粒进行计数，以每平方微米银粒数或每个视杆细胞核的银粒数表示。（摘要截取自250字）
