Pourcho R G, Goebel D J
J Comp Neurol. 1985 Mar 22;233(4):473-80. doi: 10.1002/cne.902330406.
Golgi techniques were combined with electron microscopic autoradiography to identify four subpopulations of amacrine cell in the cat retina which accumulate (3H)glycine. These subpopulations include types A3, A4, A7(AII), and A8 amacrine cells. All are narrow-field cells with dendritic spreads of less than 100 micron. Quantification of silver grains showed that each subpopulation exhibits a consistent affinity for (3H)glycine. Type A8 cells were found to have the greatest affinity with normalized grain densities of 0.88-1.0 grains/micron 2 on a scale in which 1.0 represents the most heavily labeled cell. Type A4 cells were moderately labeled with grain densities ranging from 0.40 to 0.68 grains/micron 2. A7(AII) and A3 amacrines were lightly labeled with grain densities of 0.33-0.35 grains/micron 2 and 0.28-0.30 grains/micron 2, respectively.
高尔基体技术与电子显微镜放射自显影术相结合，以鉴定猫视网膜中积累（3H）甘氨酸的四种无长突细胞亚群。这些亚群包括A3、A4、A7（AII）和A8型无长突细胞。所有这些都是窄视野细胞，其树突扩散小于100微米。银颗粒的定量分析表明，每个亚群对（3H）甘氨酸都表现出一致的亲和力。发现A8型细胞具有最大的亲和力，归一化颗粒密度为0.88 - 1.0颗粒/微米2，在此尺度上1.0代表标记最重的细胞。A4型细胞被中度标记，颗粒密度范围为0.40至0.68颗粒/微米2。A7（AII）和A3无长突细胞被轻度标记，颗粒密度分别为0.33 - 0.35颗粒/微米2和0.28 - 0.30颗粒/微米2。
