Yamashita Shun-ichi, Oku Masahide, Sakai Yasuyoshi
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwake, Sakyo-ku, Kyoto 606-8502, Japan.
Autophagy. 2007 Jan-Feb;3(1):35-7. doi: 10.4161/auto.3311. Epub 2007 Jan 28.
We recently showed that, in the yeast Pichia pastoris, an ergosterol glucoside synthesizing enzyme, Atg26, is recruited to the precursor of the pexophagic structure, micropexophagic membrane apparatus (MIPA), under the regulation of phosphatidylinositol 4'-monophosphate (PI4P)-signaling during pexophagy. Atg26 was found to harbor a novel PI4P-binding motif, the GRAM domain. Both lipids, PI4P and sterol glucoside, synthesized by PpPik1 and PpAtg26, respectively, were necessary for pexophagy, in the step where the MIPA was formed. In this addendum, we review these findings, and speculate on the mechanistic and physiological implications of the functions of these lipids during the autophagic process.
我们最近发现，在酵母毕赤酵母中，一种麦角固醇葡萄糖苷合成酶Atg26，在噬pexophagy过程中，在磷脂酰肌醇4'-单磷酸（PI4P）信号的调控下，被招募到pexophagic结构的前体，即微pexophagic膜装置（MIPA）。Atg26被发现含有一个新的PI4P结合基序，即GRAM结构域。分别由PpPik1和PpAtg26合成的两种脂质，PI4P和固醇葡萄糖苷，在MIPA形成的步骤中对pexophagy是必需的。在本附录中，我们回顾了这些发现，并推测了这些脂质在自噬过程中的功能的机制和生理意义。
