创建目标并进行分析！：协助布鲁克Reflex IV和Ultraflex II仪器进行成像质谱分析的新软件。

CreateTarget and Analyze This!: new software assisting imaging mass spectrometry on Bruker Reflex IV and Ultraflex II instruments.

作者信息

Clerens Stefan, Ceuppens Ruben, Arckens Lutgarde

机构信息

Laboratory of Neuroplasticity and Neuroproteomics, Katholieke Universiteit Leuven, Naamsestraat 59, B-3000 Leuven, Belgium.

出版信息

Rapid Commun Mass Spectrom. 2006;20(20):3061-6. doi: 10.1002/rcm.2698.

DOI:10.1002/rcm.2698

PMID:16969765

Abstract

Two new software programs are presented which enable matrix-assisted laser desorption/ionisation imaging mass spectrometry (MALDI-IMS) on Bruker Reflex and Ultraflex instruments. The first program, CreateTarget, creates a high density raster with the dimensions of the tissue section, and converts these parameters into a virtual target plate file that can be imported in the normal mass spectrometer control software. Following automated spectrum acquisition, the second program, Analyze This!, converts the array of spectra into an Analyze 7.5 image format that can be read by image analysis software. These two tools are sufficient to allow IMS, and offer a valid alternative to commercially available software. CreateTarget and Analyze This! are available free of charge.

摘要

介绍了两个新的软件程序，它们能够在布鲁克Reflex和Ultraflex仪器上进行基质辅助激光解吸/电离成像质谱分析（MALDI-IMS）。第一个程序CreateTarget生成与组织切片尺寸相同的高密度光栅，并将这些参数转换为虚拟靶板文件，该文件可导入常规质谱仪控制软件。在自动采集光谱后，第二个程序Analyze This! 将光谱阵列转换为可由图像分析软件读取的Analyze 7.5图像格式。这两个工具足以进行成像质谱分析，并为市售软件提供了有效的替代方案。CreateTarget和Analyze This! 可免费获取。