Sugrue M M, Brugge J S, Marshak D R, Greengard P, Gustafson E L
Department of Microbiology, SUNY, Stony Brook 11794.
J Neurosci. 1990 Aug;10(8):2513-27. doi: 10.1523/JNEUROSCI.10-08-02513.1990.
Neurons express high levels of a variant form of the c-src gene product, denoted pp60c-src(+), which contains a 6 amino acid insert in the amino-terminal half of the c-src protein. We have determined the localization of pp60c-src(+) in neurons using an affinity-purified anti-peptide antibody, referred to as affi-SB12, that exclusively recognizes this neuron-specific form of the c-src gene product. Using affi-SB12, we examined the distribution of pp60c-src(+) by immunoperoxidase staining of sections through adult rat brains, pp60c-src(+) was widely distributed in rat brain and appeared to be differentially expressed in subpopulations of neurons. The majority of immunoreactive neurons was found in the mesencephalon, cerebellum, pons, and medulla. Telencephalic structures that contained substantial populations of pp60c-src(+)-immunoreactive neurons included layer V of the cerebral cortex and the ventral pallidum. Within individual neurons, pp60c-src(+) immunoreactivity was localized to the cell soma and dendritic processes, while labeling of axons and nerve terminals (puncta) was not as readily detected. Dense accumulations of immunoreactive axons were rare, being most prominent in portions of the inferior and superior olive, and in the spinal trigeminal nucleus. While the regional distribution of pp60c-src(+) immunoreactivity does not correlate with any specific neuronal cell type or first messenger system, this unique pattern of expression of pp60c-src(+) suggests the existence of a previously uncharacterized functional organization within the brain. Furthermore, the localization of this neuron-specific tyrosine kinase in functionally important areas of the nerve cell, namely, dendritic processes, axons, and nerve terminals, suggests that pp60c-src(+) may regulate pleiotropic functions in specific classes of neurons in the adult central nervous system.
神经元表达高水平的c-src基因产物的一种变体形式，称为pp60c-src(+)，它在c-src蛋白的氨基末端一半含有一个6个氨基酸的插入序列。我们使用一种亲和纯化的抗肽抗体（称为affi-SB12）确定了pp60c-src(+)在神经元中的定位，该抗体专门识别c-src基因产物的这种神经元特异性形式。使用affi-SB12，我们通过对成年大鼠脑切片进行免疫过氧化物酶染色来检查pp60c-src(+)的分布，pp60c-src(+)在大鼠脑中广泛分布，并且似乎在神经元亚群中差异表达。大多数免疫反应性神经元见于中脑、小脑、脑桥和延髓。含有大量pp60c-src(+)免疫反应性神经元的端脑结构包括大脑皮层第V层和腹侧苍白球。在单个神经元内，pp60c-src(+)免疫反应性定位于细胞体和树突过程，而轴突和神经末梢（点状）的标记则不太容易检测到。免疫反应性轴突的密集聚集很少见，在上下橄榄核的部分以及脊髓三叉神经核中最为明显。虽然pp60c-src(+)免疫反应性的区域分布与任何特定的神经元细胞类型或第一信使系统均无关联，但pp60c-src(+)这种独特的表达模式表明大脑中存在一种以前未被描述的功能组织。此外，这种神经元特异性酪氨酸激酶在神经细胞的功能重要区域，即树突过程、轴突和神经末梢中的定位表明，pp60c-src(+)可能在成年中枢神经系统的特定类别的神经元中调节多效性功能。
