Sobue K, Kanda K
Department of Neurochemistry and Neuropharmacology, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1988 Dec 30;157(3):1383-9. doi: 10.1016/s0006-291x(88)81028-3.
By immunocytochemical and biochemical techniques, we observed the localization and expression of pp60c-src in nerve growth factor (NGF)-treated PC12 cells. Immunostaining of pp60c-src is detected in the neuronal soma and the tips of neurites (growth cones). Immunofluorescence in the neurites is less significant. High-resolution microscopy reveals that the location of pp60c-src in growth cone is in good agreement with the adhesive site of growth cone to the substratum. The pp60c-src kinase activity and the pp60c-src protein level increase 3.1- to 3.5-fold and 2.0-fold during differentiation of PC12 cells, respectively. The pp60c-src levels in the neurite fraction are also higher than those in the neuronal soma fraction. These results support the immunocytochemical finding that pp60c-src is localized in growth cones of differentiated PC12 cells. Furthermore, we discuss the possible role of pp60c-src in growth cone.
通过免疫细胞化学和生化技术，我们观察了神经生长因子（NGF）处理的PC12细胞中pp60c-src的定位和表达。在神经元胞体和神经突尖端（生长锥）检测到pp60c-src的免疫染色。神经突中的免疫荧光不太明显。高分辨率显微镜显示，pp60c-src在生长锥中的位置与生长锥与基质的粘附位点高度一致。在PC12细胞分化过程中，pp60c-src激酶活性和pp60c-src蛋白水平分别增加3.1至3.5倍和2.0倍。神经突部分的pp60c-src水平也高于神经元胞体部分。这些结果支持了免疫细胞化学的发现，即pp60c-src定位于分化的PC12细胞的生长锥中。此外，我们讨论了pp60c-src在生长锥中的可能作用。
