Shekhonin B V, Guriev S B, Irgashev S B, Koteliansky V E
Institute of Experimental Cardiology, USSR Cardiology Research Center, Moscow.
J Mol Cell Cardiol. 1990 May;22(5):533-41. doi: 10.1016/0022-2828(90)90955-2.
Distribution of fibrinogen/fibrin and fibronectin in regions of experimental myocardial infarction were studied by the immunofluorescence technique. Distinct from normal myocardium 3 and 12 to 24 h after coronary artery ligation infiltration of necrotized cardiomyocytes by fibrinogen/fibrin and plasma fibronectin was detected. Fibrinogen/fibrin and plasma fibronectin constitute "primary matrix" of granulation tissue. On the third day after experimental infarction, synthesis of cellular fibronectin begins. Its content in the extracellular matrix (ECM of granulation tissue significantly increases on days 7 to 15. The amount of fibronectin in the ECM of developing scar tissue dramatically decreases 30 days after infarction, Fibrinogen/fibrin was continually identified in granulation tissue in zones of myocardial infarction. However, its amount in the ECM of developing scar tissue gradually decreased.
采用免疫荧光技术研究了纤维蛋白原/纤维蛋白和纤连蛋白在实验性心肌梗死区域的分布。与正常心肌不同，在冠状动脉结扎后3小时以及12至24小时，可检测到坏死心肌细胞中有纤维蛋白原/纤维蛋白和血浆纤连蛋白浸润。纤维蛋白原/纤维蛋白和血浆纤连蛋白构成肉芽组织的“初级基质”。实验性梗死第三天，细胞纤连蛋白开始合成。在第7至15天，其在细胞外基质（肉芽组织的细胞外基质）中的含量显著增加。梗死30天后，正在形成的瘢痕组织的细胞外基质中的纤连蛋白量急剧减少。在心肌梗死区域的肉芽组织中持续可检测到纤维蛋白原/纤维蛋白。然而，其在正在形成的瘢痕组织的细胞外基质中的量逐渐减少。
