正常人主动脉和大动脉内膜以及动脉粥样硬化过程中载脂蛋白B、纤维蛋白原/纤维蛋白和纤连蛋白的可视化。

Visualization of apo B, fibrinogen/fibrin, and fibronectin in the intima of normal human aorta and large arteries and during atherosclerosis.

作者信息

Shekhonin B V, Tararak E M, Samokhin G P, Mitkevich O V, Mazurov A V, Vinogradov D V, Vlasik T N, Kalantarov G F, Koteliansky V E

机构信息

Institute of Experimental Cardiology, U.S.S.R. Cardiology Research Center, Moscow.

出版信息

Atherosclerosis. 1990 Jun;82(3):213-26. doi: 10.1016/0021-9150(90)90043-i.

DOI:10.1016/0021-9150(90)90043-i

PMID:2198029

Abstract

Apolipoprotein B (apo B), fibrinogen/fibrin, blood platelets, factor VIII-related antigen of the blood coagulation system, and smooth muscle cells (SMC) were identified in the intima of normal and atherosclerotic human aorta and large arteries by the indirect immunofluorescence technique. Fibrinogen/fibrin was revealed by a monoclonal antibody (monAb) against the C-terminal region of human fibrinogen A alpha-chain. Fibronectin was visualized by monAb to the cellular form and against an epitope shared by different fibronectin subunit variants. In normal intima, fatty streaks, small amounts of fibrinogen/fibrin together with large amounts of apo B were observed. Fibronectin detected by two types of monAb was not found in extracellular matrix (ECM), whereas cellular fibronectin encircled SMC. According to the data obtained, fibrinogen/fibrin accumulates in plaques as a result of intramural thrombus incorporation, blood insudation, intramural haemorrhage, and in or around cells, apparently macrophages.

摘要

通过间接免疫荧光技术在正常和动脉粥样硬化的人类主动脉及大动脉内膜中鉴定出载脂蛋白B（apo B）、纤维蛋白原/纤维蛋白、血小板、凝血系统的Ⅷ因子相关抗原和平滑肌细胞（SMC）。纤维蛋白原/纤维蛋白由针对人纤维蛋白原Aα链C末端区域的单克隆抗体（monAb）显示。纤连蛋白通过针对细胞形式和不同纤连蛋白亚基变体共有的表位的单克隆抗体进行可视化。在正常内膜中，观察到脂肪条纹、少量纤维蛋白原/纤维蛋白以及大量apo B。通过两种单克隆抗体检测到的纤连蛋白在细胞外基质（ECM）中未发现，而细胞纤连蛋白环绕着SMC。根据所获得的数据，纤维蛋白原/纤维蛋白通过壁内血栓形成、血液渗出、壁内出血以及在细胞内或细胞周围（明显是巨噬细胞）的积聚而在斑块中积累。