[内源性一氧化碳对犬阴茎海绵体平滑肌功能的体外影响]
[Effect of endogenous carbon monoxide on the smooth muscle function of dog penile corpus cavernosum in vitro].
作者信息
Guo Yu-Gang, Qin Wen-Bo, Song Wen-Jun, Wang Shu-Qiu
机构信息
Department of Urology, First Affiliated Hospital Jiamusi, Heilongjiang 154002, China.
出版信息
Zhonghua Nan Ke Xue. 2006 Aug;12(8):685-8.
OBJECTIVE
To study the effect of endogenous carbon monoxide (CO) on the smooth muscle function of the dog penile corpus cavernosum in vitro.
METHODS
Tissue bioassay was used to measure the corpus cavernosum muscle contraction and relaxation. The production of CO was induced in the corpus cavernosum smooth muscle, and the effect of CO on the penile corpus cavernosum smooth muscle pre-contracted by phenylephrine (PE) was determined.
RESULTS
Chlorinous hemoglobin could relax the smooth muscle stripes pre-contracted by 10 micromol/L PE. A dose-dependent relaxation was observed. The relaxation responses by 10 -100 micromol/L chlorinous hemoglobin were significant compared with the control group (P < 0. 01). The pretreatment of the muscle stripes with ZnPP-IX or methylthioninium significantly reduced the relaxing effect of chlorinous hemoglobin (P < 0.01).
CONCLUSION
The relaxing effect of endogenous CO on the smooth muscle of the penile corpus cavernosum depends on the concentration of endogenous CO. The underlying mechanism may involve the pathway from CO to cGMP production.
目的
研究内源性一氧化碳（CO）对犬阴茎海绵体平滑肌功能的体外影响。
方法
采用组织生物测定法测量海绵体肌肉的收缩和舒张。诱导海绵体平滑肌产生CO，并测定CO对由去氧肾上腺素（PE）预收缩的阴茎海绵体平滑肌的影响。
结果
氯高铁血红蛋白可使由10微摩尔/升PE预收缩的平滑肌条舒张。观察到剂量依赖性舒张。与对照组相比，10 - 100微摩尔/升氯高铁血红蛋白的舒张反应显著（P < 0.01）。用ZnPP-IX或亚甲蓝对肌条进行预处理可显著降低氯高铁血红蛋白的舒张作用（P < 0.01）。
结论
内源性CO对阴茎海绵体平滑肌的舒张作用取决于内源性CO的浓度。其潜在机制可能涉及从CO到环鸟苷酸（cGMP）生成的途径。
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