Alves Glaucie Jussilane, Vismari Luciana, Florio Jorge Camilo, Palermo-Neto João
Laboratory of Applied Pharmacology and Toxicology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
Neurosci Res. 2006 Oct;56(2):172-9. doi: 10.1016/j.neures.2006.06.012. Epub 2006 Sep 12.
The present study was designed to evaluate the effects of cohabitation for 11 days with a sick conspecific on hypothalamic levels and turnover of noradrenaline NA (experiment 1) and on neutrophil oxidative burst and phagocytosis in mice (experiment 2). Female mice were divided into two groups: control and experimental. One mouse of each control pair was kept undisturbed and called "companion of health partner" (CHP). One animal of each experimental pair of mice was inoculated with 5 x 10(6) Ehrlich tumor cells i.p., and the other, the subject of this study, was called "companion of sick partner" (CSP). In experiment 3, CHP and CSP mice were treated with diazepam (1.0 mg/kg) or with control solution (vehicle of diazepam, 1.0 mL/kg) 1h before evaluation of neutrophil activity. The CSP mice presented (1) decreased levels and increased turnover of hypothalamic NA; (2) decreased neutrophil oxidative burst after PMA or Staphylococcus aureus induction; (3) decreased percentage and intensity of neutrophil phagocytosis. In CSP mice, diazepam induced no changes in neutrophil oxidative burst or intensity of phagocytosis, but abolished almost completely the percentage of neutrophils performing phagocytosis. These data were discussed in the light of possible neuroimmune interactions.
本研究旨在评估与患病同种动物同居11天对下丘脑去甲肾上腺素（NA）水平及更新率（实验1）以及对小鼠中性粒细胞氧化爆发和吞噬作用的影响（实验2）。雌性小鼠分为两组：对照组和实验组。每组对照对中的一只小鼠不受干扰，称为“健康伙伴同伴”（CHP）。每组实验对中的一只小鼠腹腔注射5×10⁶个艾氏瘤细胞，另一只即本研究的对象，称为“患病伙伴同伴”（CSP）。在实验3中，在评估中性粒细胞活性前1小时，对CHP和CSP小鼠用安定（1.0mg/kg）或对照溶液（安定溶剂，1.0mL/kg）进行处理。CSP小鼠表现出：（1）下丘脑NA水平降低且更新率增加；（2）在佛波酯（PMA）或金黄色葡萄球菌诱导后中性粒细胞氧化爆发降低；（3）中性粒细胞吞噬作用的百分比和强度降低。在CSP小鼠中，安定未引起中性粒细胞氧化爆发或吞噬作用强度的变化，但几乎完全消除了进行吞噬作用的中性粒细胞的百分比。根据可能的神经免疫相互作用对这些数据进行了讨论。
