Tomiyoshi M Y, Sakai M, Baleeiro R B, Stankevicius D, Massoco C O, Palermo-Neto J, Barbuto J A M
Laboratory of Tumor Immunology, Department of Immunology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes 1730, CEP: 05508-900 Cidade Universitária, São Paulo, SP, Brazil.
Brain Behav Immun. 2009 May;23(4):558-67. doi: 10.1016/j.bbi.2009.02.006. Epub 2009 Feb 20.
This study evaluated the effects of cohabitation with a B16F10 melanoma-bearer cage mate on behavior and immune functions in mice. Five different experiments were conducted. In each of them, the female mice were divided into two groups: control and experimental. One mouse of each control pair was kept undisturbed and called "companion of health partner" (CHP). One mouse of each experimental pair was inoculated with B16F10 cells and the other, the subject of this study, was called "companion sick partner" (CSP). On Day 20 of cohabitation, behavior and immune parameters from CHP and CSP mice were analyzed. In comparison to the CHP, the CSP mice: (1) presented an increased general locomotion in the open field and a decreased exploration time and number of entries in the plus-maze open arms; (2) had an enhanced expression of the CD80 costimulatory molecule on Iab(+)CD11c(+) spleen cells, but no differences were found on lymph nodes cells; (3) presented an altered differentiation of bone marrow cells in the presence of GM-CSF, IL-4, and LPS in vitro, resulting in a lower percentage of Iab(+)CD80(+) cells; (4) had a deficit in the establishment of a Delayed Type of Hypersensitivity to ovalbumin, which was associated to an in vitro proliferation of an IL-10-producing lymphocyte subpopulation after ovalbumin stimulation. Corticosterone levels detected on Day 20 of cohabitation were similar in CHP and CSP mice. It is shown here that DCs phenotype in mice is affected by conditions associated with behavioral alterations indicative of an anxiety-like state induced by the cohabitation with a tumor-bearer conspecific. This phenomenon occurred probably through a nondependent corticosterone mechanism.
本研究评估了与携带B16F10黑色素瘤的同笼伙伴同居对小鼠行为和免疫功能的影响。进行了五项不同的实验。在每项实验中，雌性小鼠被分为两组：对照组和实验组。每组对照组中的一只小鼠不受干扰，称为“健康伙伴同伴”（CHP）。每组实验组中的一只小鼠接种B16F10细胞，另一只，即本研究的对象，称为“患病伙伴同伴”（CSP）。在同居第20天，分析CHP和CSP小鼠的行为和免疫参数。与CHP相比，CSP小鼠：（1）在旷场试验中总体活动增加，在十字迷宫开放臂中的探索时间和进入次数减少；（2）在Iab(+)CD11c(+)脾细胞上共刺激分子CD80的表达增强，但在淋巴结细胞上未发现差异；（3）在体外存在GM-CSF、IL-4和LPS的情况下，骨髓细胞分化发生改变，导致Iab(+)CD80(+)细胞百分比降低；（4）对卵清蛋白的迟发型超敏反应建立存在缺陷，这与卵清蛋白刺激后产生IL-10的淋巴细胞亚群的体外增殖有关。同居第20天检测到的皮质酮水平在CHP和CSP小鼠中相似。此处表明，小鼠中的树突状细胞表型受与行为改变相关的条件影响，这些行为改变表明与携带肿瘤的同种个体同居会诱发类似焦虑的状态。这种现象可能通过非依赖皮质酮的机制发生。
