Alves Glaucie Jussilane, Vismari Luciana, Palermo-Neto João
Laboratory of Applied Pharmacology and Toxicology, School of Veterinary Medicine, University of São Paulo, São Paulo, Brazil.
Neuroimmunomodulation. 2007;14(6):297-303. doi: 10.1159/000121284. Epub 2008 Mar 17.
The present study was designed to evaluate the effects of mice cohabitation with a sick conspecific cage mate on peritoneal macrophage activity and on resistance to Ehrlich tumor growth. Female mice housed in pairs were divided into control and experimental groups. One mouse of each control pair was inoculated with NaCl (0.1 ml/10 g) intraperitoneally and the other, called 'companion of healthy partner' (CHP), was kept undisturbed. One animal of each experimental pair of mice was inoculated with 5.0 x 10(6) Ehrlich tumor cells intraperitoneally and the other, the subject of this study, was called 'companion of sick partner' (CSP). Peritoneal macrophages were removed from CSP and CHP mice to analyze resident macrophage activity (experiment 1), macrophage activity after Mycobacterium bovis (experiment 2) or Ehrlich tumor cells (experiment 3) in vivo inoculations. The resistance of CSP and CHP mice to Ehrlich tumor growth was also analyzed (experiment 4). Differences between groups were not found on resident macrophage activity. However, Onco-BCG- and Ehrlich tumor-activated macrophages from CSP mice presented a decreased intensity and percentage of phagocytosis and an increased respiratory burst in the presence of Staphylococcus aureus stimulation in vitro. CSP animals at the same time displayed a decreased resistance to Ehrlich tumor growth. These data were discussed in light of a possible psychological stress effect imposed by the housing condition on mice's peritoneal macrophage activity and, as a consequence, on their resistance to Ehrlich tumor growth.
本研究旨在评估与患病同种笼伴同居的小鼠对腹腔巨噬细胞活性以及对艾氏肿瘤生长抵抗力的影响。成对饲养的雌性小鼠被分为对照组和实验组。对照组的每对小鼠中，一只腹腔注射0.1ml/10g的NaCl，另一只称为“健康伙伴的同伴”（CHP），不做处理。实验组的每对小鼠中，一只腹腔接种5.0×10(6)个艾氏肿瘤细胞，另一只即本研究的对象，称为“患病伙伴的同伴”（CSP）。从CSP和CHP小鼠中分离出腹腔巨噬细胞，以分析驻留巨噬细胞活性（实验1）、牛分枝杆菌（实验2）或艾氏肿瘤细胞（实验3）体内接种后的巨噬细胞活性。还分析了CSP和CHP小鼠对艾氏肿瘤生长的抵抗力（实验4）。在驻留巨噬细胞活性方面未发现组间差异。然而，在体外金黄色葡萄球菌刺激下，来自CSP小鼠的经Onco - BCG和艾氏肿瘤激活的巨噬细胞吞噬作用的强度和百分比降低，呼吸爆发增加。同时，CSP动物对艾氏肿瘤生长的抵抗力降低。根据饲养条件对小鼠腹腔巨噬细胞活性可能产生的心理应激效应，以及由此对其对艾氏肿瘤生长抵抗力的影响，对这些数据进行了讨论。
