Hobbs Marcia M, Lapple Dana M, Lawing Lisa F, Schwebke Jane R, Cohen Myron S, Swygard Heidi, Atashili Julius, Leone Peter A, Miller William C, Seña Arlene C
Department of Medicine, University of North Carolina at Chapel Hill, 27599, USA.
J Clin Microbiol. 2006 Nov;44(11):3994-9. doi: 10.1128/JCM.00952-06. Epub 2006 Sep 13.
Trichomonas vaginalis infection in men is an important cause of nongonococcal urethritis. Effective detection of the parasite in men using culture requires examination of multiple specimens. We compared culture and PCR-enzyme-linked immunosorbent assay in urethral swabs, urine, and semen for T. vaginalis detection in male sexual partners of women with trichomoniasis identified by wet mount and culture. Trichomonads were detected by at least one positive test in 205/280 men (73.2%) who submitted at least one specimen for culture and PCR. Whereas InPouch TV culture detected only 46/205 cases (22.5%), PCR detected 201/205 (98.0%). Urethral swab cultures from men with urethritis were more likely to be positive with shorter incubation than specimens from men without urethritis. T. vaginalis was detected more often in men with wet-mount-positive partners. Even with a sensitive PCR assay, reliable detection of T. vaginalis in male partners required multiple specimens. The majority of male sexual partners in this study were infected, emphasizing the importance of partner evaluation and treatment.
男性阴道毛滴虫感染是引起非淋菌性尿道炎的一个重要原因。采用培养法有效检测男性体内的该寄生虫需要对多个标本进行检查。我们比较了在尿道拭子、尿液和精液中采用培养法及聚合酶链反应-酶联免疫吸附测定法检测阴道毛滴虫的情况，这些标本均来自经湿片法和培养法确诊为滴虫病的女性的男性性伴侣。在提交了至少一份标本用于培养和聚合酶链反应检测的280名男性中，有205名（73.2%）至少通过一项阳性检测发现了滴虫。而InPouch TV培养法仅检测出205例中的46例（22.5%），聚合酶链反应检测出205例中的201例（98.0%）。与无尿道炎男性的标本相比，尿道炎男性的尿道拭子培养物在较短培养时间下更有可能呈阳性。在性伴侣湿片法检测呈阳性的男性中，阴道毛滴虫的检出率更高。即使采用灵敏的聚合酶链反应检测法，要可靠地检测男性性伴侣中的阴道毛滴虫也需要多个标本。本研究中的大多数男性性伴侣都受到了感染，这凸显了对性伴侣进行评估和治疗的重要性。
