Van Der Pol Barbara, Kraft Colleen S, Williams James A
Division of Infectious Diseases, Indiana University Scool of Medicine, Indianapolis, IN 46202, USA.
J Clin Microbiol. 2006 Feb;44(2):366-73. doi: 10.1128/JCM.44.2.366-373.2006.
Trichomonas vaginalis PCR using reagents from a commercially available assay for Chlamydia trachomatis and Neisseria gonorrhoeae was evaluated for detection of infection in women and men attending a sexually transmitted disease clinic. Evaluations included three primer sets, endocervical swabs, vaginal swabs and urine, and various storage conditions. The TVK3/TVK7 primer set was optimal in our hands with sensitivities ranging from 69.5 to 96.8%. In all comparisons, T. vaginalis PCR performed better than routine diagnostics using microscopy for women and culture for men (P > 0.05). The assay performed well for all sample types tested, and vaginal swabs were stable for up to 7 days at ambient temperature. Using samples prepared for, and reagents from, the C. trachomatis-N. gonorrhoeae PCR assay allowed incorporation of T. vaginalis PCR diagnosis into routine clinical testing.
利用一种市售沙眼衣原体和淋病奈瑟菌检测试剂盒中的试剂进行阴道毛滴虫聚合酶链反应（PCR），以评估在一家性传播疾病诊所就诊的女性和男性中该感染的检测情况。评估包括三种引物组、宫颈拭子、阴道拭子和尿液，以及各种储存条件。在我们的实验中，TVK3/TVK7引物组最为理想，灵敏度范围为69.5%至96.8%。在所有比较中，阴道毛滴虫PCR检测对女性的效果优于常规显微镜诊断，对男性的效果优于培养法（P>0.05）。该检测方法对所有测试样本类型均表现良好，阴道拭子在室温下可稳定保存长达7天。使用为沙眼衣原体-淋病奈瑟菌PCR检测准备的样本和试剂，能够将阴道毛滴虫PCR诊断纳入常规临床检测。
