Ffrench M, Magaud J P, Arzounian M, Souchier C, Charrin C, Bryon P A
Laboratory of Analytic Cytology, Faculty of Medicine, Lyon, France.
Cell Tissue Kinet. 1990 Jul;23(4):251-60. doi: 10.1111/j.1365-2184.1990.tb01122.x.
In some cases of acute lymphoblastic leukaemia (ALL) the percentage of cells in G2 + M is higher than anticipated when compared with the percentage in S phase. This increase in G2 + M, as detected by flow cytometry measurement of DNA content, may be due to an accumulation of cells, either in G2 or during the end of S phase; it may also be related to the existence of small tetraploid clones generally ignored by cytogeneticists. In order to identify possible subpopulations of cells with a DNA index greater than or equal to 2.0, we have compared the results of a cytogenetic analysis to the G2 + M values. We have also studied the distribution of S phase cells in 24 cases of ALL by incorporating 5-bromodeoxyuridine, labelling the cells by indirect immunofluorescence, and analysing them by flow cytometry after propidium iodide staining. The distribution of cells during S phase was quantified: no accumulation of cells was ever observed at the end of S phase. The question of the existence of small tetraploid clones, G2 arrested cells or cells with a G2 elongation remains open. However, we feel that it is more probable that, in this pathology, an elongation of the duration of G2 occurs.
在某些急性淋巴细胞白血病（ALL）病例中，与S期细胞百分比相比，G2 + M期细胞的百分比高于预期。通过流式细胞术测量DNA含量检测到的G2 + M期增加，可能是由于细胞在G2期或S期末期的积累；这也可能与细胞遗传学家通常忽略的小四倍体克隆的存在有关。为了识别DNA指数大于或等于2.0的可能细胞亚群，我们将细胞遗传学分析结果与G2 + M值进行了比较。我们还通过掺入5-溴脱氧尿苷、间接免疫荧光标记细胞并在碘化丙啶染色后通过流式细胞术分析，研究了24例ALL中S期细胞的分布。对S期细胞的分布进行了定量：在S期末期从未观察到细胞积累。小四倍体克隆、G2期停滞细胞或G2期延长细胞是否存在的问题仍然悬而未决。然而，我们认为在这种病理情况下，更有可能发生的是G2期持续时间延长。
