Singh Om V
Department of Pediatrics, The Johns Hopkins School of Medicine, Baltimore, MD, USA.
Proteomics. 2006 Oct;6(20):5481-92. doi: 10.1002/pmic.200600200.
Microbial-mediated attenuation of toxic aromatic pollutants offers great potential for the restoration of contaminated environments in an ecologically acceptable manner. However, incomplete biological information regarding the regulation of growth and metabolism in many microbial communities restricts progress in the site-specific mineralization process. In the postgenomic era, recent advances in MS have allowed enormous progress in proteomics and elucidated many complex biological interactions. These research forefronts are now expanding toward the analysis of low-molecular-weight primary and secondary metabolites analysis, i.e., metabolomics. The advent of 2-DE in conjunction with MS offers a promising approach to address the molecular mechanisms of bioremediation. The two fields of proteomics and metabolomics have thus far worked separately to identify proteins and primary and secondary metabolites during bioremediation. A simultaneous study combining functional proteomics and metabolomics, i.e., proteometabolomics would create a system-wide approach to studying site-specific microorganisms during active mineralization processes. This article deals with advances in environmental proteomics and metabolomics and advocates the simultaneous study of both technologies to implement cell-free bioremediation.
微生物介导的有毒芳香污染物的衰减为以生态可接受的方式修复受污染环境提供了巨大潜力。然而，许多微生物群落中关于生长和代谢调节的生物学信息不完整，限制了特定地点矿化过程的进展。在后基因组时代，质谱技术（MS）的最新进展使蛋白质组学取得了巨大进展，并阐明了许多复杂的生物相互作用。这些研究前沿目前正朝着低分子量初级和次级代谢产物分析，即代谢组学分析的方向扩展。二维电泳（2-DE）与质谱联用的出现为解决生物修复的分子机制提供了一种有前景的方法。蛋白质组学和代谢组学这两个领域迄今为止一直是分别开展工作，以鉴定生物修复过程中的蛋白质以及初级和次级代谢产物。将功能蛋白质组学和代谢组学结合起来进行同步研究，即蛋白质代谢组学，将创建一种全系统方法，用于在活跃矿化过程中研究特定地点的微生物。本文论述了环境蛋白质组学和代谢组学的进展，并提倡同时研究这两种技术以实现无细胞生物修复。
