Zhao Bing, Poh Chit Laa
Environment and Biotechnology Centre, Faculty of Life and Social Sciences, Swinburne University of Technology, Hawthorn, Victoria, Australia.
Proteomics. 2008 Feb;8(4):874-81. doi: 10.1002/pmic.200701005.
Environmental pollutants in the soil are a major concern worldwide. Bioremediation mediated by microorganisms is a highly promising technology that is environmentally friendly, safe, and effective. However, incomplete biological information regarding the cellular responses in many microbial communities restricts progress in the site-specific mineralization process. The application of proteomics in environmental bioremediation research provides a global view of the protein compositions of the microbial cells and offers a promising approach to address the molecular mechanisms of bioremediation. With the combination of proteomics, functional genomics provide an insight into global metabolic and regulatory networks that can enhance the understanding of gene functions. This article deals with the applications of functional genomics and proteomics to dissect the cellular responses to environmental stimuli, such as stress response, induction and expressions of regulatory proteins/enzymes in response to aromatic hydrocarbons and heavy metals. An understanding of the growth conditions governing the expression of the proteome (for example, enzymes and regulatory proteins of aromatic hydrocarbon degradation, energy generation pathways, transport and stress-related proteins) in a specific environment is essential for developing rational strategies for successful bioremediation.
土壤中的环境污染物是全球主要关注的问题。微生物介导的生物修复是一项极具前景的技术，它环保、安全且有效。然而，许多微生物群落中细胞反应的生物学信息不完整，限制了特定地点矿化过程的进展。蛋白质组学在环境生物修复研究中的应用提供了微生物细胞蛋白质组成的全局视图，并为解决生物修复的分子机制提供了一种有前景的方法。通过蛋白质组学与功能基因组学的结合，可以深入了解全局代谢和调控网络，从而增强对基因功能的理解。本文探讨了功能基因组学和蛋白质组学在剖析细胞对环境刺激的反应方面的应用，例如应激反应、对芳香烃和重金属的反应中调节蛋白/酶的诱导和表达。了解特定环境中控制蛋白质组表达的生长条件（例如，芳香烃降解的酶和调节蛋白、能量产生途径、转运和应激相关蛋白）对于制定成功生物修复的合理策略至关重要。
