Callaway T R, Edrington T S, Brabban A D, Keen J E, Anderson R C, Rossman M L, Engler M J, Genovese K J, Gwartney B L, Reagan J O, Poole T L, Harvey R B, Kutter E M, Nisbet D J
Food and Feed Safety Research Unit, USDA/ARS, College Station, Texas 77845, USA.
Foodborne Pathog Dis. 2006 Fall;3(3):234-44. doi: 10.1089/fpd.2006.3.234.
Escherichia coli O157:H7, Salmonella, and Listeria are foodborne pathogens of critical importance that often colonize cattle. E. coli O157:H7 can be specifically killed by lytic bacteriophage, and lytic bacteriophage treatment has been suggested as a pre-harvest intervention strategy to reduce foodborne pathogens in cattle. To date, no systematic approach to determine the incidence of E. coli O157:H7-infecting lytic bacteriophage has been published. Therefore, the current study was designed to determine (1) the incidence of E. coli O157, Salmonella spp., and Listeria and (2) the incidence of E. coli O157:H7-infecting bacteriophage in the feces of feedlot steers in commercial feedlots in the United States. Fecal samples (n=60) were collected from four feedlots in two Southern Great Plains states (total (n=240 fecal samples). Salmonella and E. coli O157:H7 were found in 3.8% and 11.7% of the fecal samples, respectively. Bacteriophage targeting E. coli O157:H7 were found in all four feedlots, in 15% of the individual fecal samples, and in 55% of the cattle pens. Our results indicate that such bacteriophage are widespread in feedlot cattle, suggesting that further research into the ecological role of bacteriophage in the gastrointestinal tract is needed.
大肠杆菌O157:H7、沙门氏菌和李斯特菌是极其重要的食源性病原体，它们常常在牛体内定植。溶菌性噬菌体能够特异性杀死大肠杆菌O157:H7，有人提出采用溶菌性噬菌体处理作为一种收获前干预策略，以减少牛体内的食源性病原体。迄今为止，尚未发表过确定感染大肠杆菌O157:H7的溶菌性噬菌体发生率的系统方法。因此，本研究旨在确定（1）美国商业饲养场中饲养场阉牛粪便中大肠杆菌O157、沙门氏菌属和李斯特菌的发生率，以及（2）感染大肠杆菌O157:H7的噬菌体的发生率。从南部大平原两个州的四个饲养场采集了粪便样本（n = 60）（总共（n = 240个粪便样本）。分别在3.8%和11.7%的粪便样本中发现了沙门氏菌和大肠杆菌O157:H7。在所有四个饲养场、15%的个体粪便样本以及55%的牛栏中都发现了靶向大肠杆菌O157:H7的噬菌体。我们的结果表明，此类噬菌体在饲养场的牛中广泛存在，这表明需要进一步研究噬菌体在胃肠道中的生态作用。
