Lin Hung-Yin, Hsu Chung-Yi, Thomas James L, Wang Shu-E, Chen Hsiao-Chi, Chou Tse-Chuan
Department of Chemical Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan, ROC.
Biosens Bioelectron. 2006 Oct 15;22(4):534-43. doi: 10.1016/j.bios.2006.07.038. Epub 2006 Sep 14.
The performance of molecularly imprinted polymers (MIPs) is of interest to researchers in the field of analytical chemistry, and in the pharmaceutical and food industries. Because the choice of the functional monomer(s) plays a key role in the selectivity of a MIP, the synthesis of an effective, tight-binding MIP can be difficult and time-consuming, involving the evaluation of the binding performance of MIPs of many different compositions. In this study, we report an express method combining molecular imprinting and microcontact printing techniques to prepare a polymer thin film as an artificial antibody. In addition to the microcontact printing technique, isothermal titration of monomers to proteins stamps was investigated to screen the functional monomer for MIPs. Finally, the importance of the choice of cross-linking monomers in MIPs was studied, and these studies suggest that monomers containing an optimal length PEG spacer give higher imprinting effectiveness. Several model antigens (lysozyme, ribonuclease A and myoglobin) were adsorbed on a cover glasses that were pretreated with hexamethyldisilazane (HMDS). These protein stamps were then contacted with different monomer solutions (cross-linking monomers) on a glass slide substrate. Photopolymerization yielded the molecularly imprinted polymer. This technique, analogous to microcontact printing, allows for the rapid, parallel synthesis of MIPs of different compositions, and requires very small volumes of monomers (ca. 4 microL). The technique also avoids potential solubility problems with the molecular targets. Of several cross-linking monomers screened, tetraethyleneglycol dimethacrylate (TEGDMA) gave the most selective lysozyme binding, while polyethyleneglycol 400 dimethacrylate (PEG400DMA) were most selective for ribonuclease A and myoglobin.
分子印迹聚合物（MIPs）的性能受到分析化学领域以及制药和食品行业研究人员的关注。由于功能单体的选择对MIP的选择性起着关键作用，因此合成有效、紧密结合的MIP可能既困难又耗时，这涉及对许多不同组成的MIP的结合性能进行评估。在本研究中，我们报告了一种结合分子印迹和微接触印刷技术的快速方法，以制备作为人工抗体的聚合物薄膜。除了微接触印刷技术外，还研究了单体与蛋白质印章的等温滴定，以筛选用于MIP的功能单体。最后，研究了MIP中交联单体选择的重要性，这些研究表明含有最佳长度聚乙二醇间隔基的单体具有更高的印迹效率。几种模型抗原（溶菌酶、核糖核酸酶A和肌红蛋白）吸附在经六甲基二硅氮烷（HMDS）预处理的盖玻片上。然后将这些蛋白质印章与载玻片基板上的不同单体溶液（交联单体）接触。光聚合产生分子印迹聚合物。这种类似于微接触印刷的技术允许快速、平行地合成不同组成的MIP，并且只需要非常少量的单体（约4微升）。该技术还避免了分子靶标的潜在溶解性问题。在筛选的几种交联单体中，四乙二醇二甲基丙烯酸酯（TEGDMA）对溶菌酶的结合选择性最高，而聚乙二醇400二甲基丙烯酸酯（PEG400DMA）对核糖核酸酶A和肌红蛋白的选择性最高。
