Böttcher Eva, Matrosovich Tatyana, Beyerle Michaela, Klenk Hans-Dieter, Garten Wolfgang, Matrosovich Mikhail
Institute of Virology, Philipps University, Hans-Meerwein str. 3, 35043 Marburg, Germany.
J Virol. 2006 Oct;80(19):9896-8. doi: 10.1128/JVI.01118-06.
Host cell proteases that cleave the hemagglutinin (HA) of influenza viruses in the human respiratory tract are still not identified. Here we cloned two human type II transmembrane serine proteases with known airway localization, TMPRSS2 and HAT, into mammalian expression vector. Cotransfection of mammalian cells with plasmids encoding HA and either protease resulted in HA cleavage in situ. Transient expression of either protease in MDCK cells enabled multicycle replication of influenza viruses in these cells in the absence of exogenous trypsin. These data suggest that TMPRSS2 and HAT are candidates for proteolytic activation of influenza viruses in vivo.
在人类呼吸道中切割流感病毒血凝素（HA）的宿主细胞蛋白酶仍未被鉴定出来。在这里，我们将两种已知在气道定位的人类II型跨膜丝氨酸蛋白酶TMPRSS2和HAT克隆到哺乳动物表达载体中。用编码HA的质粒和任一种蛋白酶共转染哺乳动物细胞，导致HA在原位被切割。在MDCK细胞中任一种蛋白酶的瞬时表达使得流感病毒在没有外源性胰蛋白酶的情况下能够在这些细胞中进行多轮复制。这些数据表明，TMPRSS2和HAT是流感病毒在体内进行蛋白水解激活的候选蛋白酶。
