Nakamura H, Moroji T, Nohara S, Nakamura H, Okada A
Department of Public Health, Kanazawa University School of Medicine, Japan.
Environ Res. 1990 Aug;52(2):155-63. doi: 10.1016/s0013-9351(05)80250-6.
In order to clarify the involvement of substance P (SP) and neurotensin (NT) neurons in the response of organisms to stress, SP-like immunoreactivity (SP-LI) and NT-like immunoreactivity (NT-LI) were determined in various regions of the rat brain following exposure to the whole-body vibration (20 Hz, 4 G, 90 min). SP-LI and NT-LI in the rat brain were measured by means of a sensitive and specific double-antibody solid-phase enzyme immunoassay. SP-LI in the frontal cortex was significantly reduced following whole-body vibration, while that in the nucleus accumbens and amygdala was significantly increased. NT-LI in both the frontal cortex and the hypothalamus of rats exposed to the whole-body vibration was significantly increased. However, striatal SP-LI and NT-LI remained unchanged. The present findings indicate that the SP and NT neuronal systems in various brain areas are involved in the response to stress. The frontal and amygdalofugal SP and hypothalamic NT neuronal systems, in particular, may have an important role in mediating the response of organisms to the whole-body vibration-induced stress.
为了阐明P物质（SP）和神经降压素（NT）神经元在机体对应激反应中的作用，在大鼠暴露于全身振动（20赫兹，4G，90分钟）后，测定了大鼠脑内不同区域的SP样免疫反应性（SP-LI）和NT样免疫反应性（NT-LI）。采用灵敏且特异的双抗体固相酶免疫分析法测定大鼠脑内的SP-LI和NT-LI。全身振动后，额叶皮质中的SP-LI显著降低，而伏隔核和杏仁核中的SP-LI显著增加。暴露于全身振动的大鼠额叶皮质和下丘脑中的NT-LI均显著增加。然而，纹状体中的SP-LI和NT-LI保持不变。目前的研究结果表明，不同脑区的SP和NT神经元系统参与了对应激的反应。特别是额叶和杏仁核传出的SP以及下丘脑的NT神经元系统，可能在介导机体对全身振动诱导的应激反应中起重要作用。
