Duncan D A, Mazur M T, Younger J B
Department of Pathology, University of Alabama, Birmingham.
Int J Gynecol Pathol. 1988;7(3):236-48.
A battery of nine lectins was applied to routinely processed endometrial biopsies to evaluate changes in glycoconjugate expression during the menstrual cycle and early gestation. Specific staining patterns emerged for glands, surface epithelium, predecidual and decidual cells, stromal granulocytes, and endothelium. Three lectins--wheat germ agglutinin (WGA), Ricinus communis-I (RCA-I), and Concanavalin ensiformis (Con-A)--showed consistent binding to epithelial and stromal cells in the menstrual cycle. WGA and RCA were bound to gland secretions whereas Con-A labeled epithelial cytoplasm. These three lectins stained occasional stromal cells early in the cycle, but WGA and RCA marked granulocytes whereas Con-A labeled predecidua in the late secretory phase. Succinylated WGA had a distribution similar to WGA but lacked its consistency and intensity. In the menstrual cycle peanut agglutinin reacted with ciliated cells. In addition, this lectin along with soybean agglutinin and Dolichos biflorus labeled gland secretions in pregnancy. The remaining lectins, Phaseolus vulgaris leucoagglutin and Ulex europaeus, marked some components, especially endothelium, but generally had less reactivity than the other lectins did. The results indicate that glycoconjugate production is correlated with the functional status of the endometrium. Presumably these glycoconjugates play a role in endometrial development necessary for nidation, and alterations in lectin distribution may reflect functional abnormalities not apparent by light microscopic study.
应用一组九种凝集素对常规处理的子宫内膜活检组织进行检测，以评估月经周期和妊娠早期糖缀合物表达的变化。腺体、表面上皮、蜕膜前细胞和蜕膜细胞、基质粒细胞及内皮细胞呈现出特定的染色模式。三种凝集素——麦胚凝集素（WGA）、蓖麻凝集素-I（RCA-I）和刀豆球蛋白（Con-A）——在月经周期中与上皮细胞和基质细胞有持续的结合。WGA和RCA与腺体分泌物结合，而Con-A标记上皮细胞质。这三种凝集素在周期早期偶尔会对基质细胞染色，但WGA和RCA标记粒细胞，而Con-A在分泌晚期标记蜕膜前细胞。琥珀酰化WGA的分布与WGA相似，但缺乏其一致性和强度。在月经周期中，花生凝集素与纤毛细胞反应。此外，这种凝集素与大豆凝集素和双花扁豆凝集素在妊娠时标记腺体分泌物。其余的凝集素，即菜豆白细胞凝集素和荆豆凝集素，标记了一些成分，尤其是内皮细胞，但一般来说其反应性比其他凝集素低。结果表明，糖缀合物的产生与子宫内膜的功能状态相关。推测这些糖缀合物在着床所需的子宫内膜发育中起作用，凝集素分布的改变可能反映了光学显微镜检查不明显的功能异常。
