Jin J-S, Yu C-P, Sun G-H, Lin Y-F, Chiang H, Chao T-K, Tsai W-C, Sheu L-F
Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROC.
Histol Histopathol. 2006 Dec;21(12):1287-93. doi: 10.14670/HH-21.1287.
To determine whether higher expression of fascin, an actin-bundling protein associated with motility, in conventional renal cell carcinoma (RCC) is associated with more advanced stages of the disease.
Immunohistochemical analysis of fascin expression was performed in tissue microarrays of 108 RCCs including 55 clear cell RCCs (CRCCs), 39 CRCCs with granular cell differentiation (GRCCs), 8 CRCCs with sarcomatoid differentiation (SRCCs) and 6 metastatic RCCs.
The expression of fascin was undetectable in normal renal tubules of all control cases. However, among the 108 RCC cases, fascin immunoreactivity was seen on the cell membrane and cytoplasm. The average immunostaining score for fascin was 128/400 in grade I, 170/400 in grade II, 207/400 in grade III, and 323/400 in grade IV RCC. The average immunostaining score of fascin was 187/400 for stage T1, 205/400 for stage T2, 288/400 for stage T3, and 355/400 for stage T4 cases of RCCs. Higher fascin scores in RCC were significantly correlated with higher T and N stages and nuclear grade. In addition, the fascin scores in GRCC (368+/-19) and SRCC (263+/-21) were significantly higher than in CRCC (95+/-18).
Our findings demonstrate for the first time that increased expression of fascin is associated with clinicopathological parameters of aggressiveness in patients with RCC. Fascin may be a novel biomarker for diagnosis and treatment of RCC.
确定在传统肾细胞癌（RCC）中，与运动性相关的肌动蛋白成束蛋白fascin的高表达是否与疾病的更晚期阶段相关。
在108例RCC的组织芯片上进行fascin表达的免疫组化分析，其中包括55例透明细胞RCC（CRCC）、39例具有颗粒细胞分化的CRCC（GRCC）、8例具有肉瘤样分化的CRCC（SRCC）和6例转移性RCC。
所有对照病例的正常肾小管中均未检测到fascin的表达。然而，在108例RCC病例中，fascin免疫反应性出现在细胞膜和细胞质上。I级RCC中fascin的平均免疫染色评分为128/400，II级为170/400，III级为207/400，IV级RCC为323/400。RCC中fascin的平均免疫染色评分在T1期为187/400，T2期为205/400，T3期为288/400，T4期RCC为355/400。RCC中较高的fascin评分与较高的T和N分期以及核分级显著相关。此外，GRCC（368±19）和SRCC（263±21）中的fascin评分显著高于CRCC（95±18）。
我们的研究结果首次表明，fascin表达增加与RCC患者侵袭性的临床病理参数相关。Fascin可能是RCC诊断和治疗的一种新型生物标志物。
