Tsai Wen-Chiuan, Sheu Lai-Fa, Nieh Shin, Yu Cheng-Ping, Sun Gung-Huan, Lin Yeh-Feng, Chen Ann, Jin Jong-Shiaw
Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
World J Urol. 2007 Mar;25(1):73-80. doi: 10.1007/s00345-006-0110-2. Epub 2006 Sep 23.
EMMPRIN and fascin are important factors in tumor invasion and progression. We tested the hypothesis that expression of EMMPRIN and fascin correlate with clinicopathological parameters of renal cell carcinoma (RCC). Immunohistochemical analysis of EMMPRIN and fascin were performed in tissue microarrays of 100 surgical specimens, including 35 clear-cell RCC (CRCC), 21 clear-cell RCC with granular differentiation (GRCC), 12 chromophobe RCC (ChRCC), 8 papillary RCC (PRCC), 9 carcinoma of the collecting duct of Bellini (CDC), 10 clear-cell RCC with sarcomatoid differentiation (SRCC), and 6 metastatic RCC. Average immunoscores of EMMPRIN were 100.8 in CRCC, 195.2 in GRCC, 298.4 in ChRCC, 219.2 in PRCC, 186.1 in CDC, 226.9 in SRCC, and 151.7 in metastatic RCC. Among all included cases, average EMMPRIN immunoscores were 84.6 in grade I, 130.4 in grade II, 184.3 in grade III, and 223.5 in grade IV. Additionally, average immunostaining scores of fascin were 53.6 in CRCC, 289.3 in GRCC, 193.3 in ChRCC, 151.8 in PRCC, 181.3 in CDC, 275.4 in SRCC, and 131.7 in metastatic RCC. Average fascin immunoscores were 59.3 in grade I, 91.6 in grade II, 130.2 in grade III, and 194.7 in grade IV. Higher EMMPRIN and fascin immunoscores also correlated significantly with TNM stages and survival rates in RCC. Significant correlation was found between EMMPRIN and fascin expression. In conclusion, higher expression of EMMPRIN and fascin correlate significantly with histological grades, clinical stages, and survival rates of RCC.
细胞外基质金属蛋白酶诱导因子（EMMPRIN）和肌动蛋白丝束蛋白（fascin）是肿瘤侵袭和进展的重要因素。我们验证了EMMPRIN和fascin的表达与肾细胞癌（RCC）临床病理参数相关的假说。在100个手术标本的组织芯片上进行了EMMPRIN和fascin的免疫组织化学分析，这些标本包括35例透明细胞肾细胞癌（CRCC）、21例伴有颗粒样分化的透明细胞肾细胞癌（GRCC）、12例嫌色细胞肾细胞癌（ChRCC）、8例乳头状肾细胞癌（PRCC）、9例Bellini集合管癌（CDC）、10例伴有肉瘤样分化的透明细胞肾细胞癌（SRCC）以及6例转移性肾细胞癌。EMMPRIN的平均免疫评分在CRCC中为100.8，GRCC中为195.2，ChRCC中为298.4，PRCC中为219.2，CDC中为186.1，SRCC中为226.9，转移性肾细胞癌中为151.7。在所有纳入病例中，I级的平均EMMPRIN免疫评分为84.6，II级为130.4，III级为184.3，IV级为223.5。此外，fascin的平均免疫染色评分在CRCC中为53.6，GRCC中为289.3，ChRCC中为193.3，PRCC中为151.8，CDC中为181.3，SRCC中为275.4，转移性肾细胞癌中为131.7。fascin的平均免疫评分在I级中为59.3，II级中为91.6，III级中为130.2，IV级中为194.7。较高的EMMPRIN和fascin免疫评分也与RCC的TNM分期和生存率显著相关。EMMPRIN和fascin的表达之间存在显著相关性。总之，EMMPRIN和fascin的高表达与RCC的组织学分级、临床分期和生存率显著相关。
