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肉食杆菌属及相关乳酸菌成员的16S rRNA序列测定与鲑鱼漫游球菌新种的描述

16S rRNA sequence determination for members of the genus Carnobacterium and related lactic acid bacteria and description of Vagococcus salmoninarum sp. nov.

作者信息

Wallbanks S, Martinez-Murcia A J, Fryer J L, Phillips B A, Collins M D

机构信息

Department of Microbiology, Agricultural and Food Research Council Institute of Food Research, Reading Laboratory, Shinfield, United Kingdom.

出版信息

Int J Syst Bacteriol. 1990 Jul;40(3):224-30. doi: 10.1099/00207713-40-3-224.

DOI:10.1099/00207713-40-3-224
PMID:1697764
Abstract

The phylogenetic interrelationships of members of the genus Carnobacterium and some atypical lactobacilli isolated from diseased salmonid fish were investigated by using reverse transcriptase sequencing of 16S rRNA. The four species Carnobacterium piscicola, Carnobacterium divergens, Carnobacterium gallinarum, and Carnobacterium mobile exhibited a high degree of sequence similarity with each other (ca. 96 to 98%) and formed a phylogenetically coherent group that was quite distinct from all other lactic acid bacteria. The sequence data clearly demonstrated that carnobacteria are phylogenetically closer to the genera Enterococcus and Vagococcus than to members of the genus Lactobacillus. The strains from fish were found to be phylogenetically related to the genus Vagococcus and represent a new species, Vagococcus salmoninarum. The type strain of Vagococcus salmoninarum is strain NCFB 2777.

摘要

利用16S rRNA的逆转录酶测序法,研究了肉食杆菌属成员以及从患病鲑科鱼类中分离出的一些非典型乳酸菌之间的系统发育关系。嗜鱼肉食杆菌、差异肉食杆菌、鸡肉食杆菌和运动肉食杆菌这四个物种彼此间表现出高度的序列相似性(约96%至98%),并形成了一个系统发育上连贯的群体,该群体与所有其他乳酸菌明显不同。序列数据清楚地表明,肉食杆菌在系统发育上与肠球菌属和漫游球菌属比与乳酸杆菌属的成员更为接近。发现来自鱼类的菌株在系统发育上与漫游球菌属相关,并代表一个新物种——鲑漫游球菌。鲑漫游球菌的模式菌株是NCFB 2777菌株。

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