Miura Tohru, Maruyama Keisuke, Shimakura Sei-Ichi, Kaiya Hiroyuki, Uchiyama Minoru, Kangawa Kenji, Shioda Seiji, Matsuda Kouhei
Laboratory of Regulatory Biology, Graduate School of Science and Engineering, University of Toyama, Gofuku, Toyama, Japan.
Neurosci Lett. 2006 Oct 30;407(3):279-83. doi: 10.1016/j.neulet.2006.08.071. Epub 2006 Sep 18.
Intracerebroventricular (ICV) and intraperitoneal (IP) administration of n-octanoic acid-modified ghrelin stimulates food intake in the goldfish. We examined the involvement of neuropeptide Y (NPY) in the orexigenic action of ghrelin using a NPY Y1-receptor antagonist, BIBP-3226. Food intake induced by ICV or IP injection of ghrelin was suppressed by ICV preinjection of BIBP-3226 for 1 h. We then examined whether ghrelin affects the expression of NPY mRNA in the goldfish brain using a real-time PCR method. ICV, but not IP, administration of ghrelin at a dose sufficient to stimulate food intake increased the expression of brain NPY mRNA obtained from 2 h after treatment. These results indicate that the orexigenic action of central ghrelin is mediated by the release of NPY in the brain with stimulating NPY synthesis, and that peripheral ghrelin also stimulates food intake via brain NPY pathway.
脑室内（ICV）和腹腔内（IP）注射正辛酸修饰的胃饥饿素可刺激金鱼的食物摄取。我们使用神经肽Y（NPY）Y1受体拮抗剂BIBP-3226研究了NPY在胃饥饿素促食欲作用中的参与情况。在ICV预先注射BIBP-3226 1小时后，ICV或IP注射胃饥饿素诱导的食物摄取受到抑制。然后，我们使用实时PCR方法研究胃饥饿素是否影响金鱼脑中NPY mRNA的表达。以足以刺激食物摄取的剂量进行ICV（而非IP）注射胃饥饿素后，处理2小时后获得的脑NPY mRNA表达增加。这些结果表明，中枢胃饥饿素的促食欲作用是通过刺激NPY合成来释放脑中的NPY介导的，并且外周胃饥饿素也通过脑NPY途径刺激食物摄取。
