Pawlak Paulina, Burren Alexander, Seitz Andreas, Pietsch Constanze
Agronomy, Bern University of Applied Sciences, Zollikofen, Bern CH-2052, Switzerland.
Division of Behavioural Ecology, Institute of Ecology and Evolution, University of Bern, Wohlenstrasse 50a, CH-3032, Hinterkappelen, Bern, Switzerland.
R Soc Open Sci. 2023 Feb 15;10(2):230040. doi: 10.1098/rsos.230040. eCollection 2023 Feb.
Our understanding of the timing of stress responses and specific roles of different regulatory pathways that drive stress responses is incomplete. In particular, the regulation of appetite genes as a consequence of exposure to different stressors has not been studied in sufficient detail in fish. Therefore, a stress trial was conducted with koi carp, aiming at identifying typical effects of stress on regulation of appetite genes. The stressors tank manipulation, air exposure and feed rewarding were chosen. The responses to these stressors were evaluated 10, 30 and 60 min after the stressors were applied. Orexigenic and anorexigenic genes were investigated in four different brain regions (telencephalon, hypothalamus, optic tectum and rhombencephalon). The results show that, apart from the typical appetite regulation in the hypothalamus, the different brain regions also display pronounced responses of appetite genes to the different stressors. In addition, several genes in the serotonergic, dopaminergic and gaba-related pathways were investigated. These genes revealed that rearing in pairs of two and opening of the tank lid affected anorexigenic genes, such as and which were not changed by air exposure or feed rewarding. Moreover, distress and eustress led to limited, but distinguishable gene expression pattern changes in the investigated brain regions.
我们对应激反应的时间以及驱动应激反应的不同调节途径的具体作用的理解并不完整。特别是，鱼类暴露于不同应激源后对食欲基因的调节尚未得到足够详细的研究。因此，对锦鲤进行了一项应激试验，旨在确定应激对食欲基因调节的典型影响。选择了应激源水槽操作、空气暴露和投喂奖励。在施加应激源后10、30和60分钟评估对这些应激源的反应。在四个不同的脑区（端脑、下丘脑、视顶盖和后脑）研究了促食欲和抑食欲基因。结果表明，除了下丘脑典型的食欲调节外，不同的脑区对不同应激源也表现出明显的食欲基因反应。此外，还研究了血清素能、多巴胺能和γ-氨基丁酸相关途径中的几个基因。这些基因表明，成对饲养和打开水槽盖子会影响抑食欲基因，如 和 ，而空气暴露或投喂奖励不会改变这些基因。此外，应激和良性应激导致所研究脑区的基因表达模式发生有限但可区分的变化。
