Graça José, Santos Sara
Instituto Superior de Agronomia, Universidade Técnica de Lisboa, 1349-017 Lisboa, Portugal.
Chem Phys Lipids. 2006 Oct;144(1):96-107. doi: 10.1016/j.chemphyslip.2006.08.001. Epub 2006 Aug 8.
The cork suberin polyester was partially depolymerized by a methanolysis reaction catalyzed by calcium hydroxide. The methanolisate was analysed by ESI-MS/MS in the form of M+Li adduct-ions. This reaction solubilized a mixture of monomers and oligomers, including a set of glycerol-derived dimeric and trimeric esters. Four types of glycerol esters were identified: monoacylglycerols of alpha,omega-diacids, of omega-hydroxyacids and of monoacids; diglycerol diesters of alpha,omega-diacids; diacylglycerols of alpha,omega-diacids; monoacylglycerols of linear dimeric esters of alpha,omega-diacids and omega-hydroxyacids. The alpha,omega-diacids and omega-hydroxyacids found as monomer residues in the glycerol esters are the main ones found as cork suberin monomers. It is concluded that suberin is a glycerol-derived lipid of polymeric dimensions. Due to the protective and insulating role that it plays in plants, suberin should be considered together with the other known glycerolipids that build up biological membranes.
软木栓质聚酯通过氢氧化钙催化的甲醇解反应发生部分解聚。甲醇解产物以M + Li加合离子的形式通过电喷雾串联质谱（ESI-MS/MS）进行分析。该反应使单体和低聚物的混合物溶解，其中包括一组甘油衍生的二聚体和三聚体酯。鉴定出四种类型的甘油酯：α,ω-二酸、ω-羟基酸和单酸的单酰基甘油；α,ω-二酸的二甘油二酯；α,ω-二酸的二酰基甘油；α,ω-二酸和ω-羟基酸的线性二聚体酯的单酰基甘油。在甘油酯中作为单体残基发现的α,ω-二酸和ω-羟基酸是软木栓质单体中发现的主要成分。得出的结论是，栓质是一种具有聚合物尺寸的甘油衍生脂质。由于它在植物中发挥的保护和绝缘作用，栓质应与构成生物膜的其他已知甘油脂一起考虑。
