Centro de Biotecnologia Agrícola e Agro-alimentar do Alentejo (CEBAL) / Instituto Politécnico de Beja (IPBeja), Beja, Portugal.
Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Universidade de Évora, Évora, Portugal.
BMC Plant Biol. 2018 Sep 17;18(1):198. doi: 10.1186/s12870-018-1403-5.
Gene activity is largely controlled by transcriptional regulation through the action of transcription factors and other regulators. QsMYB1 is a member of the R2R3-MYB transcription factor family related to secondary growth, and in particular, with the cork development process. In order to identify the putative gene targets of QsMYB1 across the cork oak genome we developed a ChIP-Seq strategy.
Results provide direct evidence that QsMY1B targets genes encoding for enzymes involved in the lignin and suberin pathways as well as gene encoding for ABCG transporters and LTPs implicated in the transport of monomeric suberin units across the cellular membrane. These results highlight the role of QsMYB1 as a regulator of lignin and suberin biosynthesis, transport and assembly.
To our knowledge, this work constitutes the first ChIP-Seq experiment performed in cork oak, a non-model plant species with a long-life cycle, and these results will contribute to deepen the knowledge about the molecular mechanisms of cork formation and differentiation.
基因活性在很大程度上通过转录因子和其他调节剂的作用来控制转录调控。QsMYB1 是与次生生长相关的 R2R3-MYB 转录因子家族的一员，特别是与软木形成过程相关。为了在栓皮栎基因组中鉴定 QsMYB1 的假定基因靶标，我们开发了一种 ChIP-Seq 策略。
结果提供了直接证据，证明 QsMY1B 靶向编码木质素和角质素途径中酶的基因，以及编码 ABCG 转运体和 LTPs 的基因，这些基因参与单体角质素单位穿过细胞膜的运输。这些结果突出了 QsMYB1 作为木质素和角质素生物合成、运输和组装的调节剂的作用。
据我们所知，这项工作是在栓皮栎中进行的第一个 ChIP-Seq 实验，栓皮栎是一种具有长生命周期的非模式植物物种，这些结果将有助于加深对软木形成和分化的分子机制的认识。
