Kanemaru K, Hasegawa M, Shimada H, Ihara Y
Second Laboratory of Clinical Physiology, Tokyo Metropolitan Institute of Gerontology, Japan.
Am J Pathol. 1990 Sep;137(3):677-87.
Here we report on a monoclonal antibody, H6-33, that labels various beta-amyloid plaques, including diffuse plaques in the formalin-fixed, paraffin-embedded section from the brain affected with Alzheimer's disease (AD), without formic acid pretreatment. H6-33 also labels some neurofibrillary tangles and all kuru plaques in Gerstmann-Sträussler-Scheinker disease. In sharp contrast, H6-33 did not stain beta amyloid in the leptomeningeal vessel. For specific staining, H6-33 required the presence of fetal calf serum and it was necessary for beta amyloid to be formalin fixed. These results suggest that a novel protein in the calf serum, CSX, binds formalin-fixed beta amyloid, followed by H6-33 binding. The detection of beta amyloid by CSX was nullified by formic acid pretreatment of the tissue section. In accordance with this, CSX reacted only with a polymer form of synthetic beta peptide after fixation, but not with native beta-protein or beta-peptide monomer. These observations strongly suggest that 1) meningovascular beta amyloid should have a beta-pleated sheet structure somewhat dissimilar to that of beta-amyloid cores; and 2) most, if not all, of beta-protein immunoreactivities of diffuse plaques in AD sections are presumably derived from small amounts of amyloid fibrils scattered in the normal-looking neurohil.
在此，我们报告一种单克隆抗体H6 - 33，它可标记各种β淀粉样斑块，包括来自阿尔茨海默病（AD）患者大脑的福尔马林固定石蜡包埋切片中的弥漫性斑块，无需甲酸预处理。H6 - 33还可标记格斯特曼 - 施特劳斯勒 - 申克病中的一些神经原纤维缠结和所有库鲁斑块。形成鲜明对比的是，H6 - 33未对软脑膜血管中的β淀粉样蛋白进行染色。为进行特异性染色，H6 - 33需要胎牛血清的存在，且β淀粉样蛋白需经福尔马林固定。这些结果表明，胎牛血清中的一种新蛋白CSX可结合福尔马林固定的β淀粉样蛋白，随后H6 - 33与之结合。组织切片经甲酸预处理后，CSX对β淀粉样蛋白的检测无效。据此，CSX仅在固定后与合成β肽的聚合物形式发生反应，而不与天然β蛋白或β肽单体发生反应。这些观察结果强烈表明：1）脑膜血管β淀粉样蛋白应具有与β淀粉样核心有些不同的β折叠结构；2）AD切片中弥漫性斑块的大部分（如果不是全部）β蛋白免疫反应性可能源自散在于看似正常的神经纤维网中的少量淀粉样纤维。
