对来自奇异梭菌的F-ATP酶膜嵌入转子环的结构研究。
Structural investigations of the membrane-embedded rotor ring of the F-ATPase from Clostridium paradoxum.
作者信息
Meier Thomas, Ferguson Scott A, Cook Gregory M, Dimroth Peter, Vonck Janet
机构信息
Max-Planck-Institute of Biophysics, Max-von-Laue-Strasse 3, D-60438 Frankfurt, Germany.
出版信息
J Bacteriol. 2006 Nov;188(22):7759-64. doi: 10.1128/JB.00934-06. Epub 2006 Sep 15.
Abstract
The Na(+)-translocating F-ATPase of the thermoalkaliphilic bacterium Clostridium paradoxum harbors an oligomeric ring of c subunits that resists dissociation by sodium dodecyl sulfate. The c ring has been isolated and crystallized in two dimensions. From electron microscopy of these c-ring crystals, a projection map was calculated to 7 A resolution. In the projection map, each c ring consists of two concentric, slightly staggered, packed rings, each composed of 11 densities representing the alpha-helices. On the basis of these results, it was determined that the F-ATPase from C. paradoxum contains an undecameric c ring.
摘要
嗜热嗜碱菌奇异梭菌的Na(+)-转运F-ATP酶含有一个c亚基寡聚环，该环可抵抗十二烷基硫酸钠的解离。c环已被分离并进行二维结晶。通过对这些c环晶体的电子显微镜观察，计算出了分辨率为7埃的投影图。在投影图中，每个c环由两个同心、略有交错堆积的环组成，每个环由11个代表α螺旋的密度组成。基于这些结果，确定奇异梭菌的F-ATP酶含有一个十一聚体c环。
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