Gurguis G N, Klein E, Mefford I N, Uhde T W
Section on Anxiety and Affective Disorders, National Institute of Mental Health, Bethesda, MD 20892.
Neuropsychopharmacology. 1990 Aug;3(4):297-303.
Nervous pointer dogs have been suggested as an animal model for pathological anxiety. In order to study possible disturbances in neurotransmitter functions in this animal model, we measured brain biogenic amines (norepinephrine, dopamine, and serotonin) and their metabolites in both nervous and normal dogs. Eight nervous and six normal dogs were behaviorally tested and later anesthetized and killed. Brains were removed and dissected while frozen using a punch technique. Samples were assayed using high-performance liquid chromatography (HPLC) with electrochemical detection. The nervous dogs had higher [NE] in the reticular formation and lower serotonin, and its metabolite 5-hydroxyindoleacetic acid, in the septal nuclei, indicating possible important differences in noradrenergic and serotonergic functions in the nervous dogs. There was a trend for lower [HVA] and [DOPAC] levels and a significantly lower [DOPAC]/[DA] ratio in the nervous dogs, suggesting decreased dopaminergic function.
易兴奋的指示犬已被提议作为病理性焦虑的动物模型。为了研究该动物模型中神经递质功能可能存在的紊乱，我们测量了易兴奋犬和正常犬脑中的生物胺（去甲肾上腺素、多巴胺和血清素）及其代谢产物。对8只易兴奋犬和6只正常犬进行行为测试，随后将它们麻醉并处死。取出大脑并在冷冻状态下使用打孔技术进行解剖。使用高效液相色谱法（HPLC）和电化学检测对样本进行分析。易兴奋犬的网状结构中去甲肾上腺素水平较高，而隔核中的血清素及其代谢产物5-羟吲哚乙酸水平较低，这表明易兴奋犬在去甲肾上腺素能和血清素能功能方面可能存在重要差异。易兴奋犬的高香草酸（HVA）和3,4-二羟基苯乙酸（DOPAC）水平有降低的趋势，且[DOPAC]/[DA]比值显著降低，提示多巴胺能功能下降。
