α-促黑素细胞激素可使环磷酸腺苷（cAMP）略有升高，但对人黑素细胞的基础黑素生成或紫外线刺激的黑素生成没有影响。

Alpha-MSH causes a small rise in cAMP but has no effect on basal or ultraviolet-stimulated melanogenesis in human melanocytes.

作者信息

Friedmann P S, Wren F, Buffey J, Macneil S

机构信息

Department of Dermatology, Royal Victoria Infirmary, Newcastle upon Tyne, U.K.

出版信息

Br J Dermatol. 1990 Aug;123(2):145-51. doi: 10.1111/j.1365-2133.1990.tb01841.x.

DOI:10.1111/j.1365-2133.1990.tb01841.x

PMID:1698093

Abstract

The effects of alpha-melanocyte stimulating hormone (alpha-MSH) were studied on levels of cyclic adenosine 3',5'-monophosphate (cAMP), melanin content and response to ultraviolet radiation (UVR) in cultured human melanocytes (HuMC). Foreskin HuMC were cultured in a hormone-supplemented system not dependent on the presence of phorbol esters. Following addition of alpha-MSH (10(-6) M) there was a rise in cAMP levels maximal between 5 and 15 min to 9.4 +/- 3.2 pM/10(5) cells, while control levels were 3.6 +/- 0.7 pM/10(5) cells. After 7 days' culture in the presence of alpha-MSH (10(-8) -10(-6) M) the melanin content increased by only 35%, whereas Forskolin (10(-5) M) induced a 9.5-fold rise in cAMP after 5 min and a 10.9-fold rise in melanin content after 7 days. When HuMC were irradiated daily for 6 days with UVR (Helarium fluorescent lamps emitting 20% UVB, 80% UVA) melanin content rose 2.7-fold (SE 0.3). This was unchanged or slightly reduced in the presence of alpha-MSH (10(-8)-10(-6) M). Parallel observations on Cloudman S91 melanoma cells showed that alpha-MSH caused only an 80% increase in melanin content after 4 days. The rise in melanin content induced by three daily UV-irradiations (2.4-fold, SE 0.5) was unchanged by alpha-MSH (10(-8)-10(-6) M). Although alpha-MSH induces a small rise in cAMP in HuMC this does not result in melanogenesis, and the response to UVR is not affected by alpha-MSH in either HuMC or S91 cells.

摘要

研究了α-黑素细胞刺激素（α-MSH）对培养的人黑素细胞（HuMC）中环磷酸腺苷（cAMP）水平、黑色素含量及对紫外线辐射（UVR）反应的影响。包皮HuMC在不依赖佛波酯存在的激素补充系统中培养。加入α-MSH（10⁻⁶ M）后，cAMP水平在5至15分钟内升至最高，达到9.4±3.2 pM/10⁵细胞，而对照水平为3.6±0.7 pM/10⁵细胞。在α-MSH（10⁻⁸ - 10⁻⁶ M）存在下培养7天后，黑色素含量仅增加35%，而福斯高林（10⁻⁵ M）在5分钟后使cAMP升高9.5倍，7天后使黑色素含量升高10.9倍。当HuMC每天用UVR（发射20%中波紫外线、80%长波紫外线的氦氖荧光灯）照射6天时，黑色素含量升高2.7倍（标准误0.3）。在α-MSH（10⁻⁸ - 10⁻⁶ M）存在下，这一结果未改变或略有降低。对Cloudman S91黑色素瘤细胞的平行观察表明，α-MSH在4天后仅使黑色素含量增加80%。每日三次紫外线照射诱导的黑色素含量升高（2.4倍，标准误0.5）不受α-MSH（10⁻⁸ - 10⁻⁶ M）影响。尽管α-MSH在HuMC中诱导cAMP小幅升高，但这并未导致黑色素生成，并且在HuMC或S91细胞中，α-MSH均不影响对UVR的反应。