Demirbag R, Yilmaz R, Gur M, Celik H, Guzel S, Selek S, Kocyigit A
Department of Cardiology, Faculty of Medicine, Harran University, Sanliurfa, Turkey.
Int J Clin Pract. 2006 Oct;60(10):1187-93. doi: 10.1111/j.1742-1241.2006.01042.x.
The purpose of this study was to assess DNA damage levels in subjects with metabolic syndrome (MetS). Sixty-five subjects with MetS and 65 controls were enrolled in this study. Levels of DNA damage, total antioxidant capacity (TAC), total peroxide and oxidative stress index (OSI) were measured. We found that DNA damage levels were significantly increased [155.5 (60-264) vs. 93.2 (0-208) arbitrary units; p < 0.001] and TAC levels were significantly decreased in MetS than in control (1.34 +/- 0.27 vs. 55 +/- 0.33 mmol Trolox equivalent/l; p < 0.001). A significant falling trend in TAC levels and a significant rising trend in DNA damage values with the increase in the number of metabolic disturbances (anova p < 0.001 for both) were observed. Total peroxide (30.9 +/- 4.9 vs. 21.3 +/- 2.5 micromol H2O2/l; p < 0.001) and OSI levels [2.4 (1.3-3.8) vs. 1.4 (0.7-2.3) arbitrary units; p < 0.001] were significantly higher in the subjects with MetS than in controls. We found significant negative correlation between DNA damage and TAC levels in MetS (r = -0.656, p < 0.001) and in control (r = -0.546, p < 0.001). In multiple linear regression analysis, age, body mass index, presence of MetS and number of the components of MetS were independent predictors of log-transformed DNA damage (p < 0.05, for all). DNA damage is increased in patients with MetS. The increase in DNA damage might be occur because of the increase in the imbalance between the production of oxidants and antioxidant defences in subjects with MetS.
本研究的目的是评估代谢综合征（MetS）患者的DNA损伤水平。本研究纳入了65例代谢综合征患者和65例对照。测量了DNA损伤水平、总抗氧化能力（TAC）、总过氧化物和氧化应激指数（OSI）。我们发现，与对照组相比，代谢综合征患者的DNA损伤水平显著升高[155.5（60 - 264）对93.2（0 - 208）任意单位；p < 0.001]，且TAC水平显著降低（1.34 ± 0.27对55 ± 0.33 mmol Trolox当量/升；p < 0.001）。随着代谢紊乱数量的增加，观察到TAC水平呈显著下降趋势，DNA损伤值呈显著上升趋势（两者方差分析p均< 0.001）。代谢综合征患者的总过氧化物（30.9 ± 4.9对21.3 ± 2.5微摩尔H2O2/升；p < 0.001）和OSI水平[2.4（1.3 - 3.8）对1.4（0.7 - 2.3）任意单位；p < 0.001]显著高于对照组。我们发现，代谢综合征患者组和对照组中DNA损伤与TAC水平之间均存在显著负相关（r = -0.656，p < 0.001；r = -0.546，p < 0.001）。在多元线性回归分析中，年龄、体重指数、代谢综合征的存在以及代谢综合征的组分数量是对数转换后DNA损伤的独立预测因素（所有p均< 0.05）。代谢综合征患者的DNA损伤增加。DNA损伤增加可能是由于代谢综合征患者体内氧化剂生成与抗氧化防御之间失衡加剧所致。
