Demirbag Recep, Yilmaz Remzi, Gur Mustafa, Kocyigit Abdurrahim, Celik Hakim, Guzel Salih, Selek Sahabettin
Harran University, Faculty of Medicine, Department of Cardiology, Sanliurfa, Turkey.
Mutat Res. 2005 Oct 15;578(1-2):298-307. doi: 10.1016/j.mrfmmm.2005.05.005. Epub 2005 Jun 27.
The aim of this study was to investigate the association between lymphocyte DNA damage and acute coronary syndromes (ACS).
The study population contained 53 patients with ACS, 48 patients with stable angina and 35 voluntary healty subjects. DNA damage was assessed by alkaline comed assay in peripheral lymphocyte and plasma levels of total antioxidant capacity (TAC) were determined using a novel automated measurement method.
In ACS patients, DNA damage was significantly higher than in patients with stable angina and control subjects (144+/-52 AU, 116+/-37, 68+/-34 AU; for three p<0.001, respectively). The TAC levels in patients with ACS were lower than the other groups (1.24+/-0.31 mmol Trolox equiv./l, 1.46+/-0.29 mmol Trolox equiv./l, p<0.05, respectively). DNA damage values in patients with acute miyocardial infarction were significantly higher than in patients with unstable angina (159.8+/-53.0 AU versus 131.8+/-48.4 AU; p<0.05, respectively). Lymphocyte DNA damage values in patients with ACS showed positive correlation with d-dimer (r=0.880, p<0.001) troponin I (r=538, p<0.001) and C-reactive protein (r=0.544, p<0.001) and negative correlation with TAC (r=-0.346, p=0.011). In multiple linear regression analysis, TAC (beta=-0.213, p=0.001) and d-dimer (beta=0.697, p<0.001) were independent predictors of DNA damage in patients with ACS.
These findings indicate that lymphocyte DNA damage level increases in patients with ACS. Elevated DNA damage may be related with plaque instability and be useful for the identification of patients with acute coronary syndromes.
本研究旨在探讨淋巴细胞DNA损伤与急性冠状动脉综合征（ACS）之间的关联。
研究人群包括53例ACS患者、48例稳定型心绞痛患者和35名健康志愿者。采用碱性彗星试验评估外周淋巴细胞中的DNA损伤，并使用一种新型自动测量方法测定血浆总抗氧化能力（TAC）水平。
ACS患者的DNA损伤显著高于稳定型心绞痛患者和对照组（分别为144±52 AU、116±37 AU、68±34 AU；三组比较，p均<0.001）。ACS患者的TAC水平低于其他组（分别为1.24±0.31 mmol Trolox当量/升、1.46±0.29 mmol Trolox当量/升，p<0.05）。急性心肌梗死患者的DNA损伤值显著高于不稳定型心绞痛患者（分别为159.8±53.0 AU和131.8±48.4 AU；p<0.05）。ACS患者的淋巴细胞DNA损伤值与D-二聚体（r=0.880，p<0.001）、肌钙蛋白I（r=0.538，p<0.001）和C反应蛋白（r=0.544，p<0.001）呈正相关，与TAC呈负相关（r=-0.346，p=0.011）。在多元线性回归分析中，TAC（β=-0.213，p=0.001）和D-二聚体（β=0.697，p<0.001）是ACS患者DNA损伤的独立预测因素。
这些发现表明ACS患者的淋巴细胞DNA损伤水平升高。DNA损伤升高可能与斑块不稳定有关，有助于识别急性冠状动脉综合征患者。
需注意，原文中“troponin I (r=538, p<0.001)”这里的r值538可能有误，一般r值在-1到1之间，推测可能是0.538之类的数值，但不影响整体翻译。
