Zeyrek Dost, Cakmak Alpay, Atas Ali, Kocyigit Abdurrahim, Erel Ozcan
Department of Pediatrics, Division of Allergy and Pulmonology, Harran University School of Medicine, Sanliurfa, Turkey.
Pediatr Allergy Immunol. 2009 Jun;20(4):370-6. doi: 10.1111/j.1399-3038.2008.00780.x. Epub 2008 Sep 16.
Increased production of reactive oxygen species leading to an imbalance between the oxidative forces and the antioxidant defense systems favoring an oxidative injury has been implicated in the pathogenesis of asthma. The aim of the study was to investigate the peripheral DNA damage, and its association with oxidative and antioxidative measurements in children with asthma bronchiale. The study population contained 42 children with asthma bronchiale and 32 healthy controls. DNA damage was assessed by alkaline comet assay in peripheral lymphocytes. Plasma levels of total antioxidant status (TAS), total peroxide concentration (LOOHs), and total oxidant status (TOS) were determined. In asthma bronchiale patients, DNA damage was significantly higher than in controls (17.9 +/- 11.8 AU vs. 1.2 +/- 2.0 AU, p < 0.001). Plasma TOS and LOOHs were higher in patients than in healthy controls (13.4 +/- 7.0 vs. 9.0 +/- 3.5, p = 0.002; 9.9 +/- 3.4 vs. 4.4 +/- 1.5, p < 0.001, respectively). Plasma TAS level in patients was higher than in healthy controls (5.5 +/- 2.5 vs. 1.0 +/- 0.6, p < 0.001). DNA damage was correlated with TOS (r = 0,616, p < 0.001). The findings indicated that lymphocyte DNA damage level increases in children with asthma bronchiale. Elevated DNA damage may be related to increased oxidative stress. However, the mechanism of this association, and whether it is direct or indirect, remains to be explored.
活性氧物质生成增加导致氧化力与抗氧化防御系统之间失衡，进而引发氧化损伤，这与哮喘的发病机制有关。本研究旨在调查支气管哮喘患儿的外周血DNA损伤及其与氧化和抗氧化指标的关系。研究对象包括42例支气管哮喘患儿和32名健康对照者。采用碱性彗星试验评估外周淋巴细胞的DNA损伤。测定血浆总抗氧化状态（TAS）、总过氧化物浓度（LOOHs）和总氧化状态（TOS）。支气管哮喘患者的DNA损伤显著高于对照组（17.9±11.8任意单位 vs. 1.2±2.0任意单位，p<0.001）。患者的血浆TOS和LOOHs高于健康对照组（分别为13.4±7.0 vs. 9.0±3.5，p = 0.002；9.9±3.4 vs. 4.4±1.5，p<0.001）。患者的血浆TAS水平高于健康对照组（5.5±2.5 vs. 1.0±0.6，p<0.001）。DNA损伤与TOS相关（r = 0.616，p<0.001）。研究结果表明，支气管哮喘患儿的淋巴细胞DNA损伤水平升高。DNA损伤增加可能与氧化应激增加有关。然而，这种关联的机制以及它是直接还是间接的，仍有待探索。
