Shakkthivel Piraman, Chen Shen-Ming
Department of Industrial Chemistry, Alagappa University, Karaikudi 630003, India.
Biosens Bioelectron. 2007 Mar 15;22(8):1680-7. doi: 10.1016/j.bios.2006.07.026. Epub 2006 Sep 18.
RuOx x nH2O film was electrochemically synthesized conveniently using cyclic voltammetric technique. The film formation was ascertained by the Electrochemical quartz crystal microbalance (EQCM) method and 45 ng of deposit per cycle was obtained. Stoichiometric ratio of the ruthenium and ruthenium oxide have been studied with different pH of phosphate buffer. The stability of the modified electrode in the presence of different cations and anions with different concentrations and pH were examined. Electrochemical studies have shown that the ascorbic acid (AA) and dopamine (DA) catalytic oxidation on ruthenium oxide modified electrode (RME) with a span of 300 mV separation even in the presence of uric acid (UA) with a large decrease in their respective over potential compared with bare glassy carbon electrode (GC). Accidentally, the reversible redox properties of the AA have been expediently studied on the RME using cyclic voltammetry and this peculiarity was interrogated through rotating ring disc electrode (RRDE) experiments. RRDE experiment results are conformed to the CV studies result and thus reversible redox property of AA have been reiterated. Amperometric detection under stirred condition up to approximately 0.8mM of AA and DA was carried out at free of electrode fouling. Interestingly, the regeneration of used RME electrode even after many consequent analysis, 100% was obtained.
采用循环伏安技术方便地电化学合成了RuOx·nH2O薄膜。通过电化学石英晶体微天平（EQCM）方法确定了薄膜的形成，每个循环获得45 ng的沉积物。研究了在不同pH值的磷酸盐缓冲液中钌与氧化钌的化学计量比。考察了修饰电极在不同浓度和pH值的不同阳离子和阴离子存在下的稳定性。电化学研究表明，即使在存在尿酸（UA）的情况下，氧化钌修饰电极（RME）上的抗坏血酸（AA）和多巴胺（DA）催化氧化也有300 mV的分离跨度，与裸玻碳电极（GC）相比，它们各自的过电位大幅降低。偶然地，利用循环伏安法在RME上方便地研究了AA的可逆氧化还原性质，并通过旋转环盘电极（RRDE）实验对这一特性进行了探究。RRDE实验结果与CV研究结果一致，从而重申了AA的可逆氧化还原性质。在搅拌条件下对高达约0.8 mM的AA和DA进行了安培检测，且电极未发生污染。有趣的是，即使经过多次后续分析，使用过的RME电极仍能100%再生。
