初始次氯酸钠冲洗后MTAD抗菌持久性的降低。

Reduction in antimicrobial substantivity of MTAD after initial sodium hypochlorite irrigation.

作者信息

Tay Franklin R, Hiraishi Noriko, Schuster George S, Pashley David H, Loushine Robert J, Ounsi Hani F, Grandini Simone, Yau Joyce Y Y, Mazzoni Annalisa, Donnelly Adam, King Nigel M

机构信息

Department of Oral Biology and Maxillofacial Pathology, Medical College of Georgia, Augusta, Georgia 30912-1129, USA.

出版信息

J Endod. 2006 Oct;32(10):970-5. doi: 10.1016/j.joen.2006.03.016. Epub 2006 Jul 7.

DOI:10.1016/j.joen.2006.03.016

PMID:16982276

Abstract

Potential intrinsic tetracycline staining of intraradicular dentin has been observed when BioPure MTAD was employed as the final irrigant after initial rinsing with NaOCl. This study examined the effect of NaOCl-MTAD interaction on the antimicrobial substantivity of MTAD in dentin. Dentin cores previously irrigated with either MTAD, or in conjunction with 1.3% NaOCl as an initial irrigant were placed on blood agar plates inoculated with Escherichia faecalis at 10(5) cfu/ml. Dentin cores irrigated with 1.3% NaOCl only, and autoclaved dentin disks were used as the respective positive and negative controls. After anaerobic incubation, the mean diameter of bacterial inhibition zones formed around the MTAD group was significantly larger than the NaOCl/MTAD group, which, in turn, was not significantly different from the NaOCl positive control. Oxidation of MTAD by NaOCl resulted in the partial loss of antimicrobial substantivity in a manner similar to the peroxidation of tetracycline by reactive oxygen species.

摘要

当先用次氯酸钠（NaOCl）冲洗，然后使用BioPure MTAD作为最终冲洗剂时，已观察到根管内牙本质可能出现四环素内在染色。本研究检测了NaOCl与MTAD的相互作用对MTAD在牙本质中抗菌持久性的影响。将先前用MTAD冲洗过的牙本质核，或先用1.3% NaOCl作为初始冲洗剂冲洗后再用MTAD冲洗的牙本质核，置于接种有每毫升10(5) 菌落形成单位（cfu）粪肠球菌的血琼脂平板上。仅用1.3% NaOCl冲洗的牙本质核和经高压灭菌的牙本质盘分别用作阳性和阴性对照。厌氧培养后，MTAD组周围形成的细菌抑制圈平均直径显著大于NaOCl/MTAD组，而NaOCl/MTAD组与NaOCl阳性对照相比无显著差异。NaOCl对MTAD的氧化导致抗菌持久性部分丧失，其方式类似于活性氧对四环素的过氧化作用。