Kho Patricia, Baumgartner J Craig
Oregon Health and Science University, OHSU School of Dentistry, Portland, 97201, USA.
J Endod. 2006 Jul;32(7):652-5. doi: 10.1016/j.joen.2005.11.004.
The purpose of this investigation was to compare the antimicrobial efficacy of irrigating with 1.3% NaOCl/Biopure MTAD versus irrigation with 5.25% NaOCl/15% EDTA in the apical 5 mm of roots infected with Enterococcus faecalis. Bilaterally matched human teeth were sterilized and inoculated with E. faecalis. After chemomechanical root canal preparation, the root-ends were resected and pulverized in liquid nitrogen to expose E. faecalis in dentinal tubules or other recesses away from the main root canal system. The number of colony forming units (CFU) of E. faecalis per mg was determined from the pulverized root-ends. No significant differences were seen (t = 0.70, p = 0.495) between the number of colony forming units of E. faecalis for teeth irrigated with 5.25% NaOCl/15% EDTA (mean 131 +/- 291 CFU/mg) versus those teeth irrigated with 1.3% NaOCl/Biopure MTAD (mean 187 +/- 237 CFU/mg). This study demonstrated that there is no difference in antimicrobial efficacy for irrigation with 5.25% NaOCl/15% EDTA versus irrigation with 1.3% NaOCl/Biopure MTAD in the apical 5 mm of roots infected with E. faecalis.
本研究的目的是比较用1.3%次氯酸钠/生物纯MTAD冲洗与用5.25%次氯酸钠/15%乙二胺四乙酸（EDTA）冲洗对感染粪肠球菌的根尖5毫米根管的抗菌效果。将双侧匹配的人牙进行消毒并接种粪肠球菌。在进行化学机械根管预备后，切除牙根末端并在液氮中粉碎，以使粪肠球菌暴露于牙本质小管或远离主根管系统的其他凹陷处。从粉碎的牙根末端测定每毫克粪肠球菌的菌落形成单位（CFU）数量。用5.25%次氯酸钠/15% EDTA冲洗的牙齿（平均131±291 CFU/毫克）与用1.3%次氯酸钠/生物纯MTAD冲洗的牙齿（平均187±237 CFU/毫克）之间，粪肠球菌的菌落形成单位数量没有显著差异（t = 0.70，p = 0.495）。本研究表明，在感染粪肠球菌的根尖5毫米根管中，用5.25%次氯酸钠/15% EDTA冲洗与用1.3%次氯酸钠/生物纯MTAD冲洗的抗菌效果没有差异。
