人类中性粒细胞亚群中的一种可变免疫受体。
A variable immunoreceptor in a subpopulation of human neutrophils.
作者信息
Puellmann Kerstin, Kaminski Wolfgang E, Vogel Mandy, Nebe C Thomas, Schroeder Josef, Wolf Hans, Beham Alexander W
机构信息
Institute for Clinical Chemistry, University of Heidelberg, 68167 Mannheim, Germany.
出版信息
Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14441-6. doi: 10.1073/pnas.0603406103. Epub 2006 Sep 18.
Abstract
Neutrophils are thought to rely solely on nonspecific immune mechanisms. Here we provide molecular biological, immunological, ultrastructural, and functional evidence for the presence of a T cell receptor (TCR)-based variable immunoreceptor in a 5-8% subpopulation of human neutrophils. We demonstrate that these peripheral blood neutrophils express variable and individual-specific TCRalphabeta repertoires and the RAG1/RAG2 recombinase complex. The proinflammatory cytokine granulocyte colony-stimulating factor regulates expression of the neutrophil immunoreceptor and RAG1/RAG2 in vivo. Specific engagement of the neutrophil TCR complex protects from apoptosis and stimulates secretion of the neutrophil-activating chemokine IL-8. Our results, which also demonstrate the presence of the TCR in murine neutrophils, suggest the coexistence of a variable and an innate host defense system in mammalian neutrophils.
摘要
中性粒细胞被认为仅依赖非特异性免疫机制。在此，我们提供了分子生物学、免疫学、超微结构和功能方面的证据，证明在5%-8%的人类中性粒细胞亚群中存在基于T细胞受体（TCR）的可变免疫受体。我们证明，这些外周血中性粒细胞表达可变的、个体特异性的TCRαβ库以及RAG1/RAG2重组酶复合物。促炎细胞因子粒细胞集落刺激因子在体内调节中性粒细胞免疫受体和RAG1/RAG2的表达。中性粒细胞TCR复合物的特异性结合可保护细胞免于凋亡，并刺激中性粒细胞激活趋化因子IL-8的分泌。我们的结果还证明了小鼠中性粒细胞中存在TCR，这表明哺乳动物中性粒细胞中可变宿主防御系统和固有宿主防御系统共存。
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